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You will find all involved information from placing an
order through online to delivering samples to Macrogen.
If you have further questions,
please do not hesitate to contact us at info@macrogen.com |
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Log in |
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Access to “Make an order” |
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Complete Basic
information |
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Complete Experiment
Information by downloading Template
File or by directly typing in direct online |
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Print out |
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Enclose it with
samples |
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Ship out Samples |
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Template
Type/Format |
Sample Requirements |
Remarks |
| Plasmid |
- 100 ng/µl
- Minimum volume of 20µl |
For re-sequencing, at lease 5 µl is required |
| 16S |
- Agar Plate/Glycerol Stock
- gDNA: 30-50ng
- Minimum volume of 20µl |
N/A |
PCR Product
(Purified) |
- 50ng/µl
- Minimum volume of 20µl |
For re-sequencing, at lease 5 µl is required |
PCR Product
(Unpurified)
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- 100 ng/µl
- Minimum volume of 30µl |
N/A |
| Difficult Sequence |
- 100 ng/µl
- Minimum volume of 40µl |
N/A |
| Primer Walking |
- 8 µg(for an insert size of up to 4kb)
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Single Strand Sequencing: 1µg/1 kb
insert.
If insert size is longer than 4kb,
clone is required in an agar stab
culture status. |
| a) Sample for individual tube : |
- General glycerol stock/ Agar-stab/Agar-plate
culture at room temperature.
- 1.5µl microcentrifuge tube is recommended in a dried
(lyophilized) form or solution
..(Nuclease-free TE or distilled
water) at room temperature..
- Free re-sequencing is included |
| b) 96 Well Plate
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- 8 strip
cap is recommended in a dried (lyophilized)
form or solution (Nuclease-free TE or distilled
water)
.. at
room temperature.
- Re-sequencing is additionally charged.
** In case of Multi primers for one plate
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Only two custom primers (or two universal
primers) in tubes could be acceptable for applying this
Plate sequencing.
Note : For three or more primers, another
paired primer plate with your sample plate
should be provided. If not, your order will
be regarded as Normal sequencing
reactions.
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| c) Primer
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- Primer : 10 pmol/uL in 20µl based
on 5 samples
- Primer Tm is suitable for service : 55°C ~ 60°C
- Commonly used Universal primers are available
for free of charge (ex. M13F, T7, T3)
- Design and synthesize primers if you request.
(US $0.60/base) |
| d)
To apply Plate sequencing : |
- 1 primer/1plate service :
- Multi primers/ 1plate service :
Only
two custom primers (or two universal
primers) in tubes could be acceptable
for applying this Plate
sequencing.
Note : For three or more primers, another paired primer plate with your sample
plate should be provided. If not, your order will be regarded as Normal sequencing
reactions. |
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| - Shipping address : |
Macrogen Inc.
908 World Meridian Venture Center
#60-24,
Gasan-dong,
Geumchun-gu
Seoul 153-781, Korea
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- Free shipping will be offered
for samples of more than 20 reactions.
- Do not declare the value for custom over
USD 10 for a convenience of custom clearance.
- Clearly state the postal code (153-781)
on the airway bill for timely shipping.
- Please package your DNA samples securely
and properly in order to avoid any potential
damages in
transit
such as
evaporation or contamination of samples during
shipping.
- A printed copy of your order details must
be enclosed in your sample package
for our reference.
- Dry Ice/ICEpack/ICE should not be put in samples which are dispatched to Macrogen Inc.
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- Shipping address : |
Macrogen Europe
IWO, Kamer IA3-195, Meibergdreef 39
1105 AZ Amsterdam Zuid-oost
Netherlands
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- Please contact Macrogen Europe at europe@macrogen.com
- Please package your DNA
samples securely and properly in order to avoid any potential
damages in
transit such as evaporation or
contamination of samples during shipping.
- A printed copy of your order details
must be enclosed in your sample package
for our reference. |
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