Our NGS systems provide ultra-high throughput and broad range of whole genome sequencing including de novo assembly and resequencing using powerful combination of read-length and paired-end flexibility. Macrogen's services also offer the best project schedule and protocol to customers, which reduce overall project time and cost. We accept a variety of organisms including human, monkey, rat, mouse, microorganism (fungus, yeast, bacteria), plant etc.

Metagenome means all the genetic material present in an environmental sample including soil samples, several microenvironments within the human body and the various layers within the ocean. The purposes of metagenome sequencing are to identify the organisms present in a sample and characterize the roles in the specific environment. Macrogen provides a comprehensive view of the diversity and metabolic profile of an environmental habitat using our NGS systems.

Targeted sequencing is a sequencing method that captures specific regions of interest and sequence only those regions. This includes ChIP-seq, Custom capture seuqnecing, and human whole exome sequencing. Targeted sequencing consists of capturing and seuqncing process. Some sequencing platforms require compatible capturing tools only. Also capturing process requires previously known genome Data Base, reference sequences, or protein information prior to sequencing ana analysis. We provide a various services of targeted sequencing including chromatin immunoprecipitation sequencing(ChIP-seq), custom capture sequencing and exome sequencing. a. ChIP-seq Chromatin Immuno Precipitation (Ch-IP) sequencing is a method of sequencing Ch-IPed DNA. Using this method, it is possible to do research on specific regions of genome incorporating in such as promoters to which binding proteins interact. This sequencing service is only available for the samples that were prepared by customers. We do not provide Ch-IP preparation service. b. Custom capture sequencing Custom capture sequencing: It is a useful method of sequencing captured genome for the regions that are not involved in Human Whole Exome capture. There are three types of custom capturing sequencing; Nimblegen sequence capture 385k, 2.1M array (solid base), and Agilent sureslelct(solution base). The sequencing platform can be determined by the size of the captured. Nimblegen sequence cpature 385k array can capture up to 5Mb while Nimblegen 2.1M does 30Mb, and Agilnent sureslect can capture 3.3-6.6Mb. For Nimblegen seuqnecing capture 385k and 2.1M array need specific region information that can be found in genome.uscsc.edu. Agilent sureslect requires specific region information obtained from eArray site. These reference information should be provided from customers in order to design the customized capturing arrays. Nimblegen array design asks extra cost for the design process yet there is no cost for orders above 6 plates. Capturing tools takes about 2 months to deliver. Nimblegen Seq. Cap. 385K Array Nimblegen Seq. Cap. 385k array can catupre capacity up to 5Mb. and is compatible with both FLX and Solexa platforms. For Solexa, only single end is available. Nimblegen Seq. Cap. 2.1Mb Array Nimblegen Seq. Cap. 2.1Mb Array can capture up to 30Mb and is compatible with both FLX and Solexa platforms. For Solexa, only single end is available. Agilent SureSelect Agilent sureslect can capture up to 3.3-6.6Mb and is compatible with both Solexa and SOLiD. For Solexa, both single and paired end are available. c. Exome sequencing Human exome sequencing is availalbe with three types of capturing tools; Nimblegen Sequence Capture Human Whole Exome 2.1M Array, Nimblegen Sequence Capture EZ, and Agilent SureSelect Human All Exon Kit. Sequencing platforms can vary depending on the capturing arrays. Human whole exome region capturing is available and microRNA regions as well. Capturing tools takes about 1 month to deliver. Nimblegen Sequence Capture Human Whole Exome 2.1Mb Array Nimblegen sequence capture human whole exome 2.1Mb array is solid-based capturing tools. It consits of the same architecture with 34Mb EZ and is compatible with both FLX and Solexa as well. For Solexa, only single end is available. Using control probe to measure capture efficiency makes this array highly reliable. Nimblegen Sequence Capture EZ Nimblegen sequence capture EZ array is solid-based capturing tools. It consits of the same architecture with 34Mb EZ and is compatible with both FLX and Solexa as well. For Solexa, only paired end is available. Using control probe to measure capture efficiency makes this array highly reliable. Agilent SureSelect Human All Exon Kit Agilent sureslect human all exon kit, a solution based kit, composed of 38Mb and is compatible with both solexa and SOLiD. For solexa, only paired end is available.

Transcriptome sequencing using NGS is a fast and reliable method to identify genomic information in known and novel organisms. We provide whole mRNA transcript expression analysis (full-length mRNA and EST), novel gene discovery, identification of SNP.

Methylation in the chromosomal DNA is one of the method to regulate gene expression. To characterize methylated base is to understand one of gene regulation. In methylation experiments, applying a standard bisulfite-treatment procedure, we identify the quantitative characterization of the methylation state of each CpG dinucleotide in a given target genomic sequence. For this approach DNA is treated with sodium bisulfite, resulting in the deamination of unmethylated cytosines to uracils, while methylated cytosines remain unchanged. Subsequent PCR amplification of the converted cytosine (now uracil) results in the substitution of thymine for the uracil. Comparison of the sequence obtained from the bisulfite-treated amplicon to the published sequence enabling identification of any differential methylation. Using our NGS systems, we provide methylation sites in the chromosomal DNA.

Small RNA sequencing using NGS systems is a powerful application to identify and profile miRNA, siRNA, piRNA and other non-coding RNA on any organism. We provide rapid sequencing of small RNAs for rapid identification and quantification without cloning into bacteria.