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We, Macrogen Inc., have been equipped with a variety of Next
Generation Sequencing(NGS) platforms (GS-FLX Titanium, SOLEXA GA IIx,
SOLEXA HiSeq 2000, ABI SOLiD v.4) which enable us to enrich genome research.
With these cutting-edge platforms, Macrogen offers high-throughput DNA
sequencing service at very short time. We can ensure you the best solution
that covers current genomic research and large-scale projects.
 Download
'Next Generation Sequencing Sample Submission Guide'
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 De novo and re-sequencing |
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| Our NGS systems provide ultra-high throughput
and broad range of whole genome sequencing including de novo assembly
and re-sequencing using powerful combination of read-length and paired-end
flexibility. Macrogen's services also offer the best project schedule
and protocol to customers, which reduce overall project time and cost.
We accept a variety of organisms including human, monkey, rat, mouse,
microorganism (fungus, yeast, bacteria), plant etc. |
| Metagenomics and microbial diversity analysis |
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| Metagenome means all the genetic material
present in an environmental sample including soil samples, several microenvironments
within the human body and the various layers within the ocean. The purposes
of metagenome sequencing are to identify the organisms present in a sample
and characterize the roles in the specific environment. Macrogen provides
a comprehensive view of the diversity and metabolic profile of an environmental
habitat using our NGS systems. |
| Targeted sequencing |
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Targeted sequencing is a sequencing method
that captures specific regions of interest and sequence only those regions.
This includes ChIP-seq, Custom capture sequencing, and human whole exome
sequencing. Targeted sequencing consists of capturing and sequencing
process. Some sequencing platforms require compatible capturing tools
only. Also capturing process requires previously known genome Data Base,
reference sequences, or protein information prior to sequencing and analysis.
We provide a various services of targeted sequencing including chromatin immunoprecipitation
sequencing(ChIP-seq), custom capture sequencing and exome sequencing.
a. ChIP-seq
Chromatin Immuno Precipitation (Ch-IP) sequencing is a method of sequencing Ch-IPed
DNA. Using this method, it is possible to do research on specific regions of
genome incorporating in such as promoters to which binding proteins interact.
This sequencing service is only available for the samples that were prepared
by customers. We do not provide Ch-IP preparation service.
b. Custom capture sequencing
Custom capture sequencing: It is a useful method of sequencing captured genome
for the regions that are not involved in Human Whole Exome capture. There are
three types of custom capturing sequencing; Nimblegen sequence capture 385k,
2.1M array (solid base), and Agilent sureslelct(solution base).
The sequencing platform can be determined by the size of the captured. Nimblegen
sequence cpature 385k array can capture up to 5Mb while Nimblegen 2.1M does 30Mb,
and Agilnent sureslect can capture 3.3-6.6Mb. For Nimblegen seuqnecing capture
385k and 2.1M array need specific region information that can be found in genome.uscsc.edu.
Agilent sureslect requires specific region information obtained from eArray site.
These reference information should be provided from customers in order to design
the customized capturing arrays. Nimblegen array design asks extra cost for the
design process yet there is no cost for orders above 6 plates. Capturing tools
takes about 2 months to deliver.
Nimblegen
Seq. Cap. 385K Array
Nimblegen Seq. Cap. 385k array can catupre capacity up to 5Mb. and
is compatible with both FLX and Solexa platforms. For Solexa, only
single end is available.
Nimblegen Seq.
Cap. 2.1Mb Array
Nimblegen Seq. Cap. 2.1Mb Array can capture up to 30Mb and is compatible
with both FLX and Solexa platforms. For Solexa, only single end is
available.
Agilent SureSelect
Agilent sureslect can capture up to 3.3-6.6Mb and is compatible with
both Solexa and SOLiD. For Solexa, both single and paired end are
available. |
c. Exome sequencing
Human exome sequencing is availalbe with three types of capturing tools; Nimblegen
Sequence Capture Human Whole Exome 2.1M Array, Nimblegen Sequence Capture EZ,
and Agilent SureSelect Human All Exon Kit. Sequencing platforms can vary depending
on the capturing arrays. Human whole exome region capturing is available and
microRNA regions as well. Capturing tools takes about 1 month to deliver.
Nimblegen
Sequence Capture Human Whole
Exome 2.1Mb Array
Nimblegen sequence capture human whole exome 2.1Mb array is solid-based
capturing tools. It consits of the same architecture with 34Mb EZ
and is compatible with both FLX and Solexa as well. For Solexa, only
single end is available. Using control probe to measure capture efficiency
makes this array highly reliable.
Nimblegen Sequence Capture EZ
Nimblegen sequence capture EZ array is solid-based capturing tools.
It consits of the same architecture with 34Mb EZ and is compatible
with both FLX and Solexa as well. For Solexa, only paired end is
available. Using control probe to measure capture efficiency makes
this array highly reliable.
Agilent SureSelect
Human All Exon Kit
Agilent sureslect human all exon kit, a solution based kit, composed
of 38Mb and is compatible with both solexa and SOLiD. For solexa,
only paired end is available. |
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| cDNA / transcriptome analysis |
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| Transcriptome sequencing using NGS is
a fast and reliable method to identify genomic information in known and
novel organisms. We provide whole mRNA transcript expression analysis
(full-length mRNA and EST), novel gene discovery, identification of SNP. |
| Methylation sequencing |
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Methylation in the chromosomal DNA is
one of the methods to regulate gene expression. To characterize methylated
base is to understand one of gene regulation.
In methylation experiments, applying a standard bisulfite-treatment procedure,
we identify the quantitative characterization of the methylation state of each
CpG dinucleotide in a given target genomic sequence. For this approach DNA is
treated with sodium bisulfite, resulting in the deamination of unmethylated cytosines
to uracils, while methylated cytosines remain unchanged.
Subsequent PCR amplification of the converted cytosine (now uracil) results in
the substitution of thymine for the uracil. Comparison of the sequence obtained
from the bisulfite-treated amplicon to the published sequence enabling identification
of any differential methylation. Using our NGS systems, we provide methylation
sites in the chromosomal DNA. |
| Small RNA discovery and analysis |
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| Small RNA sequencing using NGS systems
is a powerful application to identify and profile miRNA, siRNA, piRNA
and other non-coding RNA on any organism. We provide rapid sequencing
of small RNAs for rapid identification and quantification without cloning
into bacteria. |
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EZ-Seq
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