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For over a decade, Macrogen Inc. has been dedicating to provide
genomic solutions to researchers in all over the world with its advanced
DNA sequencing technology by using 23 ABI 3730XLs.
Thanks to over 10 years long know-how and large genomics
facility, we are able to provide our sequencing service for any other individuals
or institutes at the most competitive price. From small scale sequencing
reactions to large sequencing projects, we are ready to serve you with our
pleasure.
 Macrogen Customized Sequencing Brochure Download
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 Difficult sequencing |
" Sequencing of Difficult DNA templates" is
specified for templates which are not successful in normal sequencing
condition done by BigDye v.3.1, AB.
This service has different rate of price and
it is sequenced with more customized condition and
reagent. For more information,
please 
You may not get expected sequences depending
on sample characteristics.
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| 16s rRNA |
Starting from bacterial cell, we provide all inclusive
service performing DNA extraction, PCR,
sequencing
and assemble. 1,500bp or longer sequence is guaranteed for
domain bacteria |
| 18s rRNA(ITS region) |
It is getting more popular to define species by
using sequence information. This service is to find out rRNA sequence
by sequencing DNA corresponding to the rRNA.
In case of eukaryote(ITS 5.8s), after rRNA full
sequencing, we can find out the similarity of customer's microorganism
with microogarnism in NCBI.
Starting from bacterial cell, we provide all
inclusive service performing DNA extraction, PCR,
sequencing and assemble. 1,900bp or longer sequence
is guaranteed for domain bacteria. |
ITS+26S(D1/D2 region)  |
| The sequencing is most popular method to identify the
species and you can use the sequence using Ribosomal RNA coding gene.
In case of Eukaryotes, you can get the sequence by our ITS region and
26S(D1/D2) service. This service also provides the reports that we search
sequence databases (NCBI) and calculate the statistical similarity of matches
between rRNA databases (NCBI) and the target sequence.
Please find more information as you click [here] for sample preparation. |
| Primer walking |
This service is to read 2~15kb of sequence with
PCR product or plasmid DNA that cannot be read
fully by single primer
extension sequencing. Once performing end sequencing with designated
primer by customer, we design internal primers with reference to end
sequence data.
Necessary walking continues with the same method
until the whole region is covered. About one
week is taken to read
1~1.5kb by primer walking.
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| Cloning |
Besides the main purpose of the cloning, we can
improve the sequencing results through this cloning service for contaminated
PCR products which usually show double peak in chromatogram.
This service goes like below,
- Step 1. Making plasmid by inserting a customer's
PCR products into vector (T-vector or Blunt-vector)
- Step 2. Inserting the plasmid into bacteria
cell for culture
- Step 3. Cell extraction for the plasmids
Please note that a customer shall inform us
that which type of DNA polymerase was used
in PCR
before sending samples because vector
for usage totally depends on the existence
of pfu-taq in DNA polymerase.
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| Human mtDNA Full Length Sequencing |
We provide direct sequencing of amplified 16,659 base pair human mitochondria DNA genes.
- Performing whole processes including PCR, sequencing, and assembling.
- Production of PCR products using 14 PCR primer sets, base sequence analysis using 39
sequencing primers, formation of full contig using each sequencing data.
- Time required : 14 days after sampel QC.
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| Microsatellite Analysis(VNTRs) Service |
Microsatellite Analysis(VNTRs) service encompasses
a wide variety of genotyping, DNA profiling, and mutation detection
techniques for medical, environmental, and agricultural research.
Macrogen Inc. provides the Microsatellite Analysis(VNTRs) service
based on our accumulated experience and know-how in genomics. In general,
it is used for amplified fragments' size check only. Since PCR
amplification
is not available through this service, fluorescent-labeled PCR products
should be
supplied as Microsatellite Analysis(VNTRs) samples.
Service Applications :
> Microsatellite Instability
> Amplified Fragment Length Polymorphism (AFLP)
Analysis
> Terminal Restriction Fragment Length Polymorphism
(T-RFLP) Analysis
> Relative Fluorescent Quantization - Loss of
Heterozygosity (LOH), Aneuploidy Assays,
and
Large Chromosomal Deletion Detection
> Sequence-related amplified polymorphism (SRAP)
provides a useful tool for estimation of genetic
diversity and phenetic relationships in natural
and domesticated populations.
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| PCR optimization |
This service is to find optimized annealing temperature
for high quality of PCR products from the
gDNA of our customers.
If you have no time to do PCR or you are not
confident in PCR, please let us do it.
Your sequencing result can be better when we
do PCR here. |
| SNP |
SNP analysis service is highly flexible to any
specific project requirements.
SNPs (single nucleotide polymorphisms) occurs
every 100 to 300 bases along the human genome
and account for about 90% of all genetic variations. SNPs could predispose
people to disease
or influence their response to a drug.
We offer a complete package of SNP discovery
sequencing services by this step below,
PCR optimization => PCR amplification
=> Purification => Sequencing => SNP
discovery
We provide high-throughput SNP Analysis
by using ABI3730XL, automated DNA sequencer,
and the result will be available within
approximately two (2) weeks. |
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EZ-Seq
Support
