All DNA sample(s) requirement...
-double-strand, non degraded, and containing
no particulate matter
-purified by column or gel purification protocols
-A260/280 ratio of 1.8 or above
b) RNA
All RNA sample(s) requirement...
-2100 Bioanalyzer RNA integrity Number (RIN) > 8
-28S rRNA : 18S rRNA = 2:1 ratio
DNA / RNA / Tissue Quantity
a) 454 GS FLX (in EB Buffer)
HMW(>1.5kb)
LMW(70~500bp)
Paired End
5~10µg
5µg
5~10µg
* All samples except for LMW(=50ng/µl) should be provided at a concentration
of =100ng/µl
In case of cDNA synthesis, tissue(>10g) or total RNA (>1mg) would be required.
b) Illumina Genome Analyzer
( in EB buffer)
Sample Type
Total Quantity
gDNA (for single/paired-end run)
5µg (recommended)
ChIPed DNA
10ng
mRNA sequencing-total RNA
1~10µg
mRNA sequencing-mRNA
100ng
small RNA sequencing-total RNA
1~10µg
small RNA sequencing-small RNA
entire fraction of small RNA
purified from
1~10µg of total
RNA
Macrogen Korea #60-24, Gasan-dong
Geumchen-gu
Seoul, Korea
Macrogen Europe Meibergdreef 39
1105 AZ, Amsterdam
the Netherland
Macrogen USA 9700 Great Seneca Hwy
Rockvill, MD, 20850
United States
Macrogen Japan 1-1-1 Sakuragaoka,
Setagaya-ku, Tokyo
156-8502, Japan
Copyright
2005-2011 (C) Macrogen Inc. All rights reserved.
We recommend resolution of 1024x768 or higher, and Internet explorer
of v6.0 or more advanced version for the proper display of this web
site.
EZ-Seq
Support
Download 'Next Generation Sequencing
Sample Submission Guide'
DNA / RNA Quality
