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Macrogen has served over 10 years in sequencing
field using the cutting edge technology and
delivering fast and reliable results. We
use state-of-the-art high throughput Applied
Biosystems 3730XL sequencers.
We sequence all kinds of cloned DNAs (plasmids,
cosmid, phages, BACs) as well as PCR-products.
Macrogen provides a high quality DNA Sequencing
service for academics, government, research
institutions and private companies at Very
Competitive Price.
Please contact us for any inquiry.
For more detailed explanations and recommendations
about the preparation and submission of samples,
please refer to the following link:
For information regarding to the shipping
specifications, please refer to the following
link
 Download 'Shipping Information for Value Service to Macrogen Inc. (Korea)'
Download'Shipping Information for European Express Service to Macrogen Europe
(The Netherlands)'
http://dna.macrogen.com/eng/support/seq/seq_orderguide.jsp
To order the sequencing service, please
access to our online ordering system and create an account;
http://dna.macrogen.com/eng
For payment information, please refer
to the following link:
Download 'Payment Information'
http://dna.macrogen.com/eng/support/howtopay.jsp
Contact
Sequencing Inquiry: info@macrogen.com
Payment Inquiry: payment@macrogen.com
Genome and Project sequencing inquiry: ngs@macrogen.com
Technical Support
S.H. Cha, Ph.D : chash@macrogen.co.kr
Shipping Address
(for Value Service to Macrogen Inc.)
Macrogen Inc.
908 World Meridian Venture Center
#60-24,
Gasan-dong,
Geumchun-gu
Seoul 153-781, Korea
Tel : +82-2-2113-7790
Fax : +82-2-2113-7919
E-mail : info@macrogen.com
Shipping Address (For
European Express Service to Macrogen Europe)
[Macorgen Europe (The Netherlands) ]
IWO, Kamer IA3-195
Meibergdreef 39
1105 AZ Amsterdam Zuid-oost
Netherlands
Tel : +31-(0)20-566-5472
Mobile : +31-(0)6-8148-0760
E-mail : europe@macrogen.com
|
 Single
Pass Sequencing Sample Requirements |
Template
Type/Format |
Sample Requirements |
Remarks |
| Plasmid |
- 100 ng/µl
- Minimum volume of 20µl |
For re-sequencing, at lease
5 µl is required |
| 16S |
- Agar Plate/Glycerol Stock
- gDNA: 30-50ng
- Minimum volume of 20µl |
N/A |
PCR Product
(Purified) |
- 50ng/µl
- Minimum volume of 20µl |
For re-sequencing, at lease
5 µl is required |
PCR Product
(Unpurified) |
- 100 ng/µl
- Minimum volume of 30µl |
N/A |
| Difficult Sequence |
- 100 ng/µl
- Minimum volume of 40µl |
N/A |
| Primer Walking |
- 8 µg(for an insert
size of up to 4kb)
|
Single Strand Sequencing:
1µg/1 kb
insert.
If insert size is longer than 4kb,
clone is required in an agar stab
culture status. |
|
| a)
Sample for individual tube
: |
- General glycerol
stock/ Agar-stab/Agar-plate culture
at room temperature.
- 1.5mL Microcentrifuge Tube is recommended in a dried (lyophilized)
form or solution
..(Nuclease-free
TE or distilled water) at room
temperature..
- Free re-sequencing is included |
| b)
96 Well Plate : |
- 8 strip cap
is recommended in a dried (lyophilized)
form or solution (Nuclease-free
TE or distilled water)
.. at
room temperature.
- Re-sequencing is
additionally charged.
** In case of Multi primers for one plate **
Only two custom primers (or two universal primers) in tubes could be acceptable
for applying this Plate sequencing.
* Note:
- For three or more primers, another paired primer plate with your sample….reactions.
- The well to well variation of the concentration of your samples is very important
to get
better results, please keep it as constant as possible.
- Please prepare samples to avoid
any well-to-well concentration
difference or size differencefor
quality results.
|
| c)
Primer : |
- Primer : 10
pmol/uL in 20µl based on
5 samples
- Primer Tm is suitable for service : 55°C ~ 60°C
- Commonly used Universal primers are available for free of charge
(ex. M13F, T7, T3)
- Design and synthesize primers if you request. (US $0.60/base) |
It is possible to send either a stab or glycerol
culture with the selected E.coli clone,
the purified plasmid DNA or your unpurified
or purified PCR product.
Templates and primers must be provided in
DI water or 10mM Tris buffer, not in TE |
| d) To apply Plate sequencing : |
- 1 primer/1plate service :
- Multi primers/ 1plate service :
Only
two custom primers (or two universal
primers) in tubes could be acceptable
for applying this Plate
sequencing.
Note : For three or more primers, another paired primer plate with your sample
plate should be provided. If not, your order will be regarded as Normal sequencing
reactions. |
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