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¸¶Å©·ÎÁ¨¿¡¼´Â °í°´ÆíÀǸ¦ À§ÇÏ¿© ¹«·á Bioinformatics Report Service¸¦ Á¤½ÄÀ¸·Î ·ÐĪÇÏ¿´½À´Ï´Ù.
º» ¼ºñ½º´Â Standard Sequencing °ú Customized Sequencing °ü·ÃµÈ ÁÖ¹® (16s rRNA, 18s rRNA, Primer walking, Cloning, PCR optimization,
SNP) ¿¡ ´ëÇØ Àû¿ëµÇ¸ç, ¾Æ·¡¿Í °°ÀÌ º¸´Ù Áøº¸µÈ Bioinformatics Report¸¦ ¹«·á·Î ¹Þ¾Æº¸½Ç ¼ö ÀÖ½À´Ï´Ù.
Standard Sequencing ÀÇ °æ¿ì, ÁÖ¹®ÇÏ½Ç ¶§ BI Report ¼ºñ½º¸¦ Á÷Á¢ ¼±ÅÃÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.
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| 1. Contig »ý¼º |
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| Reaction °á°ú¸¦ ¹ÙÅÁÀ¸·Î Contig¸¦ »ý¼ºÇϰí, ÇØ´ç contig¸¦ ÀÌ·ç´Â reaction alignment Á¤º¸¸¦ º¸¿©ÁÝ´Ï´Ù.
(ÆÄ¶õºÎºÐÀº reaction alignÀ» ÇÏ¿© contig »ý¼º ½Ã, ¿À¹ö·¦ µÇ´Â ºÎºÐÀ» ÀǹÌÇÕ´Ï´Ù.) |
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| <±×¸² 1>Contig align ¹× Contig chtomatogram |
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| 2. Contig Á¤º¸ |
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| »ý¼ºµÈ ContigÀÇ read length(Normal), read length(Q16), read length(Q20), GC content, contig quality Á¤º¸¸¦ È®ÀÎÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù. |
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| <±×¸² 2>Contig analysis ¹× Contig quality ±×·¡ÇÁ |
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| 3. Contig Blast °á°ú È®ÀÎ |
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| Contig¿¡ ´ëÇÑ BlastN, BlastX °á°ú¿¡¼ »óÀ§ 10°³¸¦ ÃßÃâÇÏ¿© °á°ú¸¦ ÇÑ´«¿¡ È®ÀÎÇϱ⠽±µµ·Ï Ç¥·Î Á¤¸®ÇÏ¿© Á¦°øÇÕ´Ï´Ù.
Âü°í·Î Ç¥¿¡¼ DescriptionÀ» Ŭ¸¯Çϸé ÇØ´ç °á°ú¿¡ ´ëÇÑ NCBI »çÀÌÆ®°¡ ³ªÅ¸³³´Ï´Ù. |
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| <±×¸² 3> Contig BlastN °ú BlastX °á°ú |
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| 4. GeneticTree |
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| 16s rRNA / 18s rRNA ¼ºñ½ºÀÇ °æ¿ì ±âº»ÀûÀ¸·Î GeneticTree °á°ú¸¦ Á¦°øÇØ µå¸³´Ï´Ù.
Á¦°øÇص帮´Â GeneticTree´Â contigÀÇ blastn °á°ú¸¦ ¹ÙÅÁÀ¸·Î »ý¼ºµË´Ï´Ù. |
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| <±×¸² 4> GeneticTree |
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| 5. SNP Detection |
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| SNP ¼ºñ½º¸¦ ÁÖ¹®ÇϽаæ¿ì, reactionµéÀÇ contig¸¦ »ý¼ºÇÏ¿© °í°´´Ô²²¼ Á¦°øÇØÁֽŠreference ¿Í ºñ±³ÇÏ¿© SNP, Indel ºÎºÐÀ» detection ÇØ µå¸®°í, ÇØ´ç SNP¿Í Indel¿¡ ´ëÇÏ¿© annotationÀ» Á¦°øÇØ µå¸³´Ï´Ù. ¶ÇÇÑ Contig »Ó¸¸ ¾Æ´Ï¶ó Reaction º°·Îµµ Reference ¿Í ºñ±³ÇÏ¿© Á¤º¸¸¦ Á¦°øÇØ µå¸³´Ï´Ù. |
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| <±×¸² 5-1> Contig¿Í reference align |
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| <±×¸² 5-2> SNP¿Í Indel Annotation |
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| 6. ORF(Open reading Frame) Analysis |
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| »ý¼ºµÈ Contig ³»¿¡¼ ORF Áö¿ªÀ» ºÐ¼®ÇÏ¿© ¾Æ·¡¿Í °°ÀÌ Á¤º¸¸¦ Á¦°øÇØ µå¸³´Ï´Ù.
(°í°´´Ô²²¼ ¿äûÀ» Áֽøé Bioinformatics Report¿¡ ORF ºÐ¼®À» Ãß°¡ÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.) |
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| <±×¸² 7> ORF analysis |
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| Bioinformatics Report¸¦ È®ÀÎÇϽ÷Á¸é ÁÖ¹®³»¿ª¿¡¼ BI Report ( ) ¹öưÀ» Ŭ¸¯ÇÏ½Ã¸é µË´Ï´Ù. |
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¸¶Å©·ÎÁ¨¿¡¼´Â º¸´Ù ³ªÀº ¼ºñ½º¸¦ Á¦°øÇϱâ À§ÇØ °í°´´ÔÀÇ ¼ÒÁßÇÑ ÀǰßÀ» ±â´Ù¸®°í ÀÖ½À´Ï´Ù.
Bioinformatics Report¸¦ ¹«·á·Î »ç¿ëÇØ º¸½Ã°í °³¼± »çÇ× µîÀÇ ÀǰßÀ» ³²°ÜÁÖ¼¼¿ä.
ÀǰßÀ» Áֽźв²(¼±Âø¼ø 30¸í) ´øÅ² Ä¿ÇÇ&µµ³Ó¼¼Æ®(3,200¿ø »ó´ç)¸¦ µå¸³´Ï´Ù. ¸¹Àº Âü¿© ºÎʵ右´Ï´Ù.
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| Ç×»ó °í°´´Ô²² ÃÖ»óÀÇ ¼ºñ½º¸¦ Á¦°øÇϱâ À§ÇØ ¾ðÁ¦³ª ³ë·ÂÇÏ´Â ¸¶Å©·ÎÁ¨ÀÌ µÇ°Ú½À´Ï´Ù.
°¨»çÇÕ´Ï´Ù. |
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