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마크로젠의 Next Generation Sequencing 은
Genome Sequencer
FLX , Solexa(Genome Analyzer II) , Applied Biosystems SOLiD 기기를 모두 보유
하고 있으며, de novo sequencing, Whole genome resequencing, Target DNA region,
Metagenomics, Transcriptome, RNA 분석 등 다양한 분야의 연구를 High-Throughput 으로 비용
절감과 최단 시간에 가장 만족할 만한 결과를 낼 수 있는 기술을 보유하고 있습니다. |
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 De novo and resequencing |
| Our NGS systems provide ultra-high throughput
and broad range of whole genome sequencing including de novo assembly
and resequencing using powerful combination of read-length and paired-end
flexibility. Macrogen's services also offer the best project schedule
and protocol to customers, which reduce overall project time and
cost. We accept a variety of organisms including human, monkey,
rat, mouse, microorganism (fungus, yeast, bacteria), plant etc. |
| Metagenomics and microbial diversity analysis |
| Metagenome means all the genetic material present
in an environmental sample including soil samples, several microenvironments
within the human body and the various layers within the ocean. The
purposes of metagenome sequencing are to identify the organisms
present in a sample and characterize the roles in the specific environment.
Macrogen provides a comprehensive view of the diversity and metabolic
profile of an environmental habitat using our NGS systems. |
| Targeted sequencing |
Targeted sequencing is a sequencing method that
captures specific regions of interest and sequence
only those regions. This includes ChIP-seq, Custom capture seuqnecing,
and human whole
exome sequencing. Targeted sequencing consists of
capturing and seuqncing process. Some sequencing platforms require
compatible
capturing tools only. Also capturing process requires
previously known genome Data Base, reference sequences, or protein
information
prior to sequencing ana analysis.
We provide a various services of targeted sequencing
including chromatin immunoprecipitation sequencing(ChIP-seq),
custom capture sequencing and exome sequencing.
a. ChIP-seq
Chromatin Immuno Precipitation (Ch-IP) sequencing
is a method of sequencing Ch-IPed DNA. Using
this method, it is possible to do research
on specific regions of genome incorporating
in such as promoters to which binding proteins
interact. This sequencing service is only
available for the samples that were prepared
by customers. We do not provide Ch-IP preparation
service.
b. Custom capture sequencing
Custom capture sequencing: It is a useful
method of sequencing captured genome for the
regions
that are not involved in Human Whole Exome
capture. There are three types of custom
capturing sequencing; Nimblegen sequence
capture 385k, 2.1M array (solid base), and
Agilent sureslelct(solution base).
The sequencing platform can be determined by
the size of the captured. Nimblegen sequence
cpature
385k array can capture up to 5Mb while Nimblegen
2.1M does 30Mb, and Agilnent sureslect can capture
3.3-6.6Mb. For Nimblegen seuqnecing capture 385k
and 2.1M array need specific region information
that can be found in genome.uscsc.edu. Agilent
sureslect requires specific region information
obtained from eArray site. These reference information
should be provided from customers in order to
design the customized capturing arrays. Nimblegen
array design asks extra cost for the design process
yet there is no cost for orders above 6 plates.
Capturing tools takes about 2 months to deliver.
Nimblegen Seq. Cap.
385K Array
Nimblegen Seq. Cap. 385k array can catupre
capacity up to 5Mb. and is compatible
with both FLX and Solexa platforms. For
Solexa, only single end is available.
Nimblegen Seq. Cap. 2.1Mb Array
Nimblegen Seq. Cap. 2.1Mb Array
can capture up to 30Mb and
is compatible with both FLX
and Solexa platforms. For
Solexa, only single end is
available.
Agilent SureSelect
Agilent sureslect can capture
up to 3.3-6.6Mb and is compatible
with both Solexa and SOLiD.
For Solexa, both single and
paired end are available. |
c. Exome sequencing
Human exome sequencing is availalbe with three types
of capturing tools; Nimblegen Sequence Capture Human Whole Exome 2.1M
Array, Nimblegen Sequence Capture EZ, and Agilent SureSelect Human All
Exon Kit. Sequencing platforms can vary depending on the capturing arrays.
Human whole exome region capturing is available and microRNA regions as
well. Capturing tools takes about 1 month to deliver.
Nimblegen Sequence Capture Human Whole Exome 2.1Mb
Array
Nimblegen sequence capture human whole
exome 2.1Mb array is solid-based capturing tools. It consits of
the same architecture with 34Mb EZ and is compatible with both FLX
and Solexa as well. For Solexa, only single end is available. Using
control probe to measure capture efficiency makes this array highly
reliable.
Nimblegen Sequence Capture EZ
Nimblegen sequence capture EZ
array is solid-based capturing
tools. It consits of the same architecture with 34Mb EZ and
is compatible
with both FLX and Solexa
as well. For Solexa, only paired end is available. Using control
probe to measure capture
efficiency makes this array
highly reliable.
Agilent SureSelect Human All Exon Kit
Agilent sureslect human all exon
kit, a solution based kit, composed of 38Mb and is compatible
with both solexa and SOLiD. For solexa, only paired
end is available. |
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| cDNA / transcriptome analysis |
| Transcriptome sequencing using NGS is a fast
and reliable method to identify genomic information in known and
novel organisms. We provide whole mRNA transcript expression analysis
(full-length mRNA and EST), novel gene discovery, identification
of SNP. |
| Methylation sequencing |
Methylation in the chromosomal DNA is one of
the method to regulate gene expression. To characterize methylated
base is to understand one of gene regulation.
In methylation experiments, applying a standard bisulfite-treatment
procedure, we identify the quantitative characterization of the
methylation state of each CpG dinucleotide in a given target genomic
sequence. For this approach DNA is treated with sodium bisulfite,
resulting in the deamination of unmethylated cytosines to uracils,
while methylated cytosines remain unchanged.
Subsequent PCR amplification of the converted cytosine
(now uracil) results in the substitution of thymine for the uracil.
Comparison of the sequence obtained from the bisulfite-treated amplicon
to the published sequence enabling identification of any differential
methylation. Using our NGS systems, we provide methylation sites
in the chromosomal DNA. |
| Small RNA discovery and analysis |
| Small RNA sequencing using NGS systems is a
powerful application to identify and profile miRNA, siRNA, piRNA
and other non-coding RNA on any organism. We provide rapid sequencing
of small RNAs for rapid identification and quantification without
cloning into bacteria. |
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Standard sequencing
