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¸¶Å©·ÎÁ¨ÀÇ SequencingÀº cDNA Library, Shotgun Library µî Library Á¦ÀÛ¿¡¼­ºÎÅÍ Sequencing service¿¡ À̸£´Â ÀÏ·ÃÀÇ °úÁ¤µéÀ» ½Å¼ÓÇϰí Á¤È®ÇÏ°Ô ¼öÇàÇϰí ÀÖ½À´Ï´Ù. Bioinformatics ±â¼úÀ» Á¢¸ñÇÑ Blast Search ¹× single contig ÀÇ ¿Ï¼º¿¡ À̸£±â±îÁöÀÇ ¾÷¹«µéÀ» ¼öÇàÇϸç À̿ܿ¡ Sequencing À» À§ÇØ ÇÊ¿äÇÑ Cell Culture, Colony Picking, DNA Prepa-ration, PCR ¹× Purfication, Cell Stock Á¦ÀÛ µî ´Ù¾çÇÑ ºÎ°¡¼­ºñ½ºµéµµ ÃÖ»óÀÇ ¼­ºñ½º·Î Á¦°øÇϰí ÀÖ½À´Ï´Ù.

ÀÚÁÖ ¹®ÀÇÇϽô Ç׸ñµéÀ» Á¤¸®ÇÏ¿´½À´Ï´Ù. [FAQ ¹Ù·Î°¡±â]
Difficult sequencing
ÀϹÝÀûÀÎ ½ÃÄö½ÌÁ¶°Ç(BigDye v.3.1, AB)¿¡¼­´Â ÁÁÀº °á°ú¸¦ ¾òÀ» ¼ö ¾ø´Â template¸¦ ´ë»óÀ¸·Î ÇÏ´Â ½ÃÄö½Ì ¼­ºñ½º ÀÔ´Ï´Ù. ÀϹÝÀûÀÎ ½ÃÄö½Ì°ú´Â ´Ù¸¥ Á¶°Ç°ú ½Ã¾àÀ¸·Î ÁøÇàµÇ¸ç, °¡°Ýü°è ¶ÇÇÑ ´Ù¸¨´Ï´Ù.

Á» ´õ ÀÚ¼¼ÇÑ Á¤º¸¸¦ ¿øÇϽøé [¿©±â]¸¦ ´©¸£½Ã±â ¹Ù¶ø´Ï´Ù.

*»ùÇÃÀÇ Æ¯À̼º¿¡ µû¶ó ¼º°øÀûÀÎ °á°ú¸¦ ¾òÁö ¸øÇÏ´Â °æ¿ìµµ ÀÖ½À´Ï´Ù.

16s rRNA
Bacterial cellÀ» ÀÌ¿ëÇÏ¿© ÇÙ»êÃßÃâ->PCR->Sequencing->AssembleµîÀÇ ¸ðµç °úÁ¤À» ¼öÇàÇØ µå¸®´Â ¼­ºñ½º ÀÔ´Ï´Ù.
domain BacteriaÀÇ °æ¿ì 1,300base pairsÀÌ»óÀÇ °á°ú¸¦ º¸ÀåÇÏ¿© µå¸³´Ï´Ù.
»ùÇà Áغñ´Â [¿©±â]¸¦ Ŭ¸¯ÇÏ¿© Âü°íÇØ Áֽñ⠹ٶø´Ï´Ù.

18s rRNA(ITS region)
»ý¸íüÀÇ Á¾À» µ¿Á¤ÇÏ´Â ¹æ¹ý Áß Çϳª·Î ¿°±â¼­¿­ Á¤º¸¸¦ ÀÌ¿ëÇÏ´Â ¹æ¹ýÀÌ ³Î¸® ÀÌ¿ëµÇ°í ÀÖ½À´Ï´Ù. ¿°±â¼­¿­ Á¤º¸´Â small subunit ribosome À» ±¸¼ºÇÏ´Â rRNA¸¦ ¿°±â ¼­¿­ ºÐ¼®ÇÏ¿© ÀÌ¿ëÇÕ´Ï´Ù.

ÁøÇÙ»ý¹°ÀÇ °æ¿ì 18S(ITS 5.8s) rRNA full sequencingÀ» ÅëÇØ ¿°±â¼­¿­À» ºÐ¼®ÇÏ°í ±× °á°ú¸¦
rRNA database(NCBI)¿Í ºñ±³ÇÏ¿© ´ë»ó ¹Ì»ý¹°ÀÌ ¾î¶² ¹Ì»ý¹°°ú À¯»çÇÑÁö¸¦ È®ÀÎÇÏ´Â ¼­ºñ½º ÀÔ´Ï´Ù.

»ùÇà Áغñ´Â [¿©±â]¸¦ Ŭ¸¯ÇÏ¿© Âü°íÇØ Áֽñ⠹ٶø´Ï´Ù.

ITS+26S(D1/D2 region)
»ý¸íüÀÇ Á¾À» µ¿Á¤ÇÏ´Â ¹æ¹ý Áß Çϳª·Î ¿°±â¼­¿­ Á¤º¸¸¦ ÀÌ¿ëÇÏ´Â ¹æ¹ýÀÌ ³Î¸® ÀÌ¿ëµÇ°í ÀÖ½À´Ï´Ù. ¿°±â¼­¿­ Á¤º¸´Â Ribosomal RNA À¯ÀüÀÚ¸¦ ¿°±â¼­¿­ ºÐ¼®ÇÏ¿© ÀÌ¿ëÇÕ´Ï´Ù.

ÁøÇÙ»ý¹°ÀÇ °æ¿ì ITS region ¹× 26S(D1/D2) region sequencing À» ÅëÇØ ¿°±â¼­¿­À» ºÐ¼®ÇÏ°í ±× °á°ú¸¦ rRNA database(NCBI)¿Í ºñ±³ÇÏ¿© ´ë»ó ¹Ì»ý¹°ÀÌ ¾î¶² ¹Ì»ý¹°°ú À¯»çÇÑÁö¸¦ È®ÀÎÇÏ´Â ¼­ºñ½º ÀÔ´Ï´Ù.

»ùÇà Áغñ´Â [¿©±â]¸¦ Ŭ¸¯ÇÏ¿© Âü°íÇØ Áֽñ⠹ٶø´Ï´Ù.

Primer walking
single primer extensionÀ¸·Î Çѹø¿¡ Àо ¼ö ¾ø´Â plasmid ³ª PCR productÀÇ ¿°±â¼­¿­À» ºÐ¼®ÇÏ´Â ¼­ºñ½º À̸ç, º¸Åë 2-10kb Á¤µµÀÇ ½ÃÄö½º Á¤º¸¸¦ ¾ò°íÀÚ ÇÒ¶§ ÀÌ¿ëÇÏ´Â ¹æ¹ýÀÔ´Ï´Ù.°í°´´Ô²²¼­ Á¦°øÇϽðųª ÁöÁ¤ÇØÁֽŠprimer·Î end sequencingÀ» ¼öÇàÇÑ ÈÄ ¾òÀº °á°ú¹°·ÎºÎÅÍ internal primer¸¦ µðÀÚÀÎÇÏ¿© Á¦ÀÛÇÕ´Ï´Ù. »õ·Î¿î Primer·Î °°Àº template¸¦ ¹ÝÀÀÇÏ¿© ¾òÀº °á°ú·ÎºÎÅÍ ¶Ç ´Ù½Ã ÀûÁ¤ÇÑ À§Ä¡¿¡¼­ internal primer¸¦ µðÀÚÀÎÇÏ¿© °è¼Ó ¿¬ÀåÇÕ´Ï´Ù. 1ȸ walking ¼öÇà¿¡ 4ÀÏ Á¤µµ°¡ ¼Ò¿äµÇ¸ç, ÇÑ ¹æÇâ ´ç ¾à 500bp~800bp ¿¬ÀåÀÌ °¡´ÉÇÕ´Ï´Ù.
Cloning
PCR ProductÀÇ ContaminationÀÇ °æ¿ì ¿©·¯ Á¾·ùÀÇ Product°¡ Á¸ÀçÇÏ¿© sequencing °á°ú°¡ ÁÁÁö ¾ÊÀº °æ¿ì(sequence PeakÀÇ °ãÄ¡´Â Çö»ó) CloningÀ» ÅëÇØ °³¼±ÇÒ ¼ö ÀÖ½À´Ï´Ù.

PCR Product¸¦ vector (T-vector or Blunt-vector)¿¡ »ðÀÔÇÏ¿© Plasmid ¸¦ ¸¸µì´Ï´Ù.
Plasmid¸¦ Bacteria cell¿¡ ÁÖÀÔÇÏ¿© ¹è¾çÈÄ cell extraction ÇÕ´Ï´Ù.
PCR½Ã »ç¿ëÇÏ´Â DNA polymerase(pfu-taq »ç¿ë À¯¹«)¿¡ µû¶ó vector(T-vector or Blunt-vector)¸¦ ´Ù¸£°Ô »ç¿ëÇÕ´Ï´Ù.

µû¶ó¼­ ÁÖ¹® ÀÇ·ÚÀü¿¡ »ç¿ëÇϽŠtaqÀÇ ±â´É À¯¹«¸¦ Ç¥±âÇÏ¿© Áֽñ⠹ٶø´Ï´Ù. (with pfu ¶Ç´Â without pfu)

Human mtDNA Full Length Sequencing
16,659 bpÀÇ human mitochondria DNA À¯ÀüÀÚ¸¦ ÁõÆøÇÏ¿© ¸ðµç ¿°±â¼­¿­À» ºÐ¼®ÇÏ´Â ¼­ºñ½º.

- PCR, sequencing, assemble µî ¸ðµç °úÁ¤ ¼öÇà.
- 15 PCR primer setÀ» ÀÌ¿ëÇÏ¿© PCR product »ý¼º, 40°³ sequencing primer¸¦ ÀÌ¿ëÇÏ¿© ¿°±â¼­¿­
   ºÐ¼®, °¢°¢ÀÇ seqeucning data¸¦ ÀÌ¿ëÇÏ¿© ÇϳªÀÇ full contig »ý¼º.
- ¼Ò¿ä±â°£ : Sample QC È®ÀÎ ÈÄ 14ÀÏ.



Microsatellite Analysis(VNTRs) Service
Microsatellite Analysis(VNTRs) service encompasses a wide variety of genotyping, DNA profiling, and mutation detection techniques for medical, environmental, and agricultural research. Macrogen Inc. provides the Microsatellite Analysis(VNTRs) service based on our accumulated experience and know-how in genomics. In general, it is used for amplified fragments' size check only. Since PCR
amplification is not available through this service, fluorescent-labeled PCR products should be
supplied as Microsatellite Analysis(VNTRs) samples.

Service Applications :
> Microsatellite Instability
> Amplified Fragment Length Polymorphism (AFLP) Analysis
> Terminal Restriction Fragment Length Polymorphism (T-RFLP) Analysis
> Relative Fluorescent Quantization - Loss of Heterozygosity (LOH), Aneuploidy Assays,
   and Large Chromosomal Deletion Detection
> Sequence-related amplified polymorphism (SRAP) provides a useful tool for estimation of genetic
   diversity and phenetic relationships in natural and domesticated populations.



PCR optimization
º¸³»ÁֽŠgenomic DNA¿¡¼­ PCR Product¸¦ ¸¸µé¶§ ÃÖÀûÀÇ annealing TMÀ» ã¾Æ ÃÖÀûÈ­µÈ PCR Product¸¦ »ý»êÇϵµ·Ï Á¶°ÇÀ» Àâ¾ÆÁÖ´Â ¼­ºñ½º ÀÔ´Ï´Ù.
gDNA¿¡¼­ ÃÖÀûÀÇ PCR Product°¡ »ý»êµÇ¾î¾ß ³ôÀº ǰÁúÀÇ sequencing data¸¦ ¹Þ¾Æº¸½Ç ¼ö ÀÖ½À´Ï´Ù.

SNP
SNP analysis service is highly flexible to any specific project requirements.
SNPs (single nucleotide polymorphisms) occurs every 100 to 300 bases along the human genome
and account for about 90% of all genetic variations. SNPs could predispose people to disease or influence their response to a drug.

We offer a complete package of SNP discovery sequencing services by this step below,
PCR optimization => PCR amplification => Purification => Sequencing => SNP discovery

We provide high-throughput SNP Analysis by using ABI3730XL, automated DNA sequencer, and the result will be available within approximately two (2) weeks.


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¼­¿ï½Ã ±Ýõ±¸ °¡»êµ¿ 60-24
¿ùµå¸Þ¸£µð¾Óº¥Ãļ¾ÅÍ(1Â÷) 910È£ 153-781
ÀüÈ­¹®ÀÇ : 02)2113-7070
¸¶Å©·ÎÁ¨(´ëÀüÁö»ç)
´ëÀü±¤¿ª½Ã À¯¼º±¸ °üÆòµ¿ 1359¹øÁö
Çѽſ¡½º¸ÞÄ« 436È£
ÀüÈ­¹®ÀÇ : 042)336-7080
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