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¸¶Å©·ÎÁ¨ÀÇ SequencingÀº cDNA Library, Shotgun Library µî Library Á¦ÀÛ¿¡¼­ºÎÅÍ Sequencing service¿¡ À̸£´Â ÀÏ·ÃÀÇ °úÁ¤µéÀ» ½Å¼ÓÇϰí Á¤È®ÇÏ°Ô ¼öÇàÇϰí ÀÖ½À´Ï´Ù. Bioinformatics ±â¼úÀ» Á¢¸ñÇÑ Blast Search ¹× single contig ÀÇ ¿Ï¼º¿¡ À̸£±â±îÁöÀÇ ¾÷¹«µéÀ» ¼öÇàÇϸç À̿ܿ¡ Sequencing À» À§ÇØ ÇÊ¿äÇÑ Cell Culture, Colony Picking, DNA Prepa-ration, PCR ¹× Purfication, Cell Stock Á¦ÀÛ µî ´Ù¾çÇÑ ºÎ°¡¼­ºñ½ºµéµµ ÃÖ»óÀÇ ¼­ºñ½º·Î Á¦°øÇϰí ÀÖ½À´Ï´Ù.

ÀÚÁÖ ¹®ÀÇÇϽô Ç׸ñµéÀ» Á¤¸®ÇÏ¿´½À´Ï´Ù. [FAQ ¹Ù·Î°¡±â]
- 3730xl DNA analyzer
- LIMS (Laboratory Information Management System)¸¦ ±â¹ÝÀ¸·Î sample Á¢¼öºÎÅÍ °á°ú   ¹ß¼Û±îÁö ½Ç½Ã°£ ¸ð´ÏÅ͸µ
- 24½Ã°£ ³»¿¡ °á°ú Å뺸 (¿ÀÈÄ 4½Ã ÀÌÀü ȸ»ç µµÂøºÐ¿¡ ÇÑÇÔ)
- ±âº»ÀûÀÎ ºÐ¼® ¼­ºñ½º ¹«·á ´ëÇà   (BlastN ¶Ç´Â BlastX¸¦ ÅëÇÑ ´Ü¹éÁú°ú DNA¿°±â¼­¿­ ¼öÁØ¿¡¼­ »óµ¿¼º °Ë»ö)
Template
- Plasmid : ³óµµ 100ng/§¡, Volume ±âº» 5ul+(¹ÝÀÀ¼ö*2ul)
- PCR product (purified) : ³óµµ 50ng/§¡, Volume ±âº» 5ul+(¹ÝÀÀ¼ö*2ul)
- PCR product (non-purified) : ³óµµ 100ng/§¡, Volume 20§¡
Primer
- Concentration : 5pmole/§¡ (5pM), Volume : 20§¡ (¿¹) 5 Reaction ¹ÝÀÀ½Ã : 20§¡, reaction ¼ö°¡ Ãß°¡µÉ ¶§¸¶´Ù 2§¡¾¿ Áõ°¡
- °í°´ÀÌ primer Á¦°ø ½Ã ÇÊÈ÷ template¿Í primer ºÐ¸® ¿ä¸Á
- Primer Tm : 55~60¡É, GC content : 40~60%
Normal plasmid template ±âÁØ, phred score 20 ÀÌ»ó, read length 650 bpÀÌ»ó º¸Àå
- 3730xl ¿Ï·á
- °á°ú ÆÄÀÏ (ab1, txt, pdf, phd) »ý¼º
- BlastN °Ë»ö
- ¹®ÀÚ¸Þ¼¼Áö ÀÚµ¿ ¹ß¼Û
- Data ¹ß¼Û ¹× ¾÷·Îµå

Data Á¢¼ö ¹× process ÁøÇà°úÁ¤¿¡ ´ëÇØ¼­ ½Ç½Ã°£ ¸ð´ÏÅ͸µÀÌ °¡´ÉÇÕ´Ï´Ù.
¶ÇÇÑ °í°´´ÔÀÇ ÆíÀǸ¦ À§ÇØ Data ºÐ¼® ¿Ï·á¿Í µ¿½Ã¿¡ ¹®ÀÚ¸Þ¼¼Áö°¡ ¹ß¼ÛµÇ¿À´Ï LIMS ¹× E-mail¿¡¼­ °á°úÈ®ÀÎ ¹× download ÇÒ ¼ö ÀÖ½À´Ï´Ù. (±âº»ÀûÀÎ ºÐ¼® ¼­ºñ½º)


Standard-seq single
°¡Àå ÀϹÝÀûÀÎ single primer extension ¼­ºñ½º ÀÔ´Ï´Ù.
Plasmid³ª PCR product¸¦ ÁÖÇüÀ¸·Î ÇÏ°í °í°´´Ô²²¼­ ÁöÁ¤ÇϽŠprimer¸¦ ÀÌ¿ëÇØ ±¸°£ÀÇ ¿°±â¼­¿­ ºÐ¼®À» ¼öÇàÇÏ´Â ¼­ºñ½º ÀÔ´Ï´Ù.
1Primer/1Plate
" 1 Primer / 1 Plate"´Â ´ÙÀ½ÀÇ µÎ°¡Áö Á¶°ÇÀÌ ¸ðµÎ ¸¸Á·ÇÏ¿©¾ß¸¸ ÇÕ´Ï´Ù.
(1) »ùÇÃÀº ¹Ýµå½Ã 96 or 384 plate¿¡ ´ã°Ü ÀÖ¾î¾ß ÇÕ´Ï´Ù.
(2) °¢ plate´ç ÇѰ³ÀÇ ÇÁ¶óÀ̸Ӹ¸ »ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù.

- »ùÇÃÀ̸§Àº ÀÔ·ÂÇÏ½Ç Çʿ䰡 ¾øÀ¸¸ç, plate À̸§¸¸À» ÀÔ·ÂÇÏ´Â °ÍÀ¸·Î ÃæºÐÇÕ´Ï´Ù.
  µû¶ó¼­ ½ÃÄö½Ì °á°ú´Â [Plate_name]-[Well_position]_[Primer_name].ab1 ÀÇ Çü½ÄÀ¸·Î ¹ß¼ÛµË´Ï´Ù.

[Plate_name]-[Well_position]_[Primer_name].ab1
ex)
HCVNS5B-H05_SP6.ab1
BRCA2-D24_T7.ab1
2 primers / 1 plate
" Plate Sequencing"Àº ´ÙÀ½ÀÇ ¼¼°¡Áö Á¶°ÇÀÌ ¸ðµÎ ¸¸Á·ÇÏ¿©¾ß¸¸ ÇÕ´Ï´Ù.
(1) »ùÇÃÀº ¹Ýµå½Ã 96 plate¿¡ ´ã°Ü ÀÖ¾î¾ß ÇÕ´Ï´Ù.
(2) °¢ plate´ç µÎ°³±îÁöÀÇ ÇÁ¶óÀÌ¸Ó »ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù.
(3) ¼¼ °³ ÀÌ»óÀÇ ÇÁ¶óÀ̸Ӹ¦ »ç¿ëÇÏ½Ç °æ¿ì, »ùÇà plate¿Í pair°¡ µÇµµ·Ï primer plate¸¦ ¸¸µé¾î ÁÖ¼Å¾ß ÇÕ´Ï´Ù.

- »ùÇÃÀ̸§°ú ÇÁ¶óÀÌ¸Ó À̸§À» ¸ðµÎ ÀÔ·ÂÇØ ÁÖ¼Å¾ß Çϸç,
  ½ÃÄö½Ì °á°ú´Â [sample_name]-[Primer_name].ab1 ÀÇ Çü½ÄÀ¸·Î ¹ß¼ÛµË´Ï´Ù.
[Plate_name]-[Well_position]_[Primer_name].ab1
ex)
HCVNS5B-H05_SP6.ab1
BRCA2-D24_T7.ab1
¸¶Å©·ÎÁ¨(º»»ç)
¼­¿ï½Ã ±Ýõ±¸ °¡»êµ¿ 60-24
¿ùµå¸Þ¸£µð¾Óº¥Ãļ¾ÅÍ(1Â÷) 910È£ 153-781
ÀüÈ­¹®ÀÇ : 02)2113-7070
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´ëÀü±¤¿ª½Ã À¯¼º±¸ °üÆòµ¿ 1359¹øÁö
Çѽſ¡½º¸ÞÄ« 436È£
ÀüÈ­¹®ÀÇ : 042)336-7080
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