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¸¶Å©·ÎÁ¨ÀÇ SequencingÀº cDNA Library, Shotgun Library µî
Library Á¦ÀÛ¿¡¼ºÎÅÍ Sequencing service¿¡ À̸£´Â
ÀÏ·ÃÀÇ °úÁ¤µéÀ» ½Å¼ÓÇϰí Á¤È®ÇÏ°Ô ¼öÇàÇϰí ÀÖ½À´Ï´Ù.
Bioinformatics ±â¼úÀ» Á¢¸ñÇÑ Blast Search ¹× single contig
ÀÇ ¿Ï¼º¿¡ À̸£±â±îÁöÀÇ ¾÷¹«µéÀ» ¼öÇàÇϸç À̿ܿ¡ Sequencing À» À§ÇØ ÇÊ¿äÇÑ Cell Culture, Colony Picking,
DNA Prepa-ration, PCR ¹×
Purfication, Cell Stock Á¦ÀÛ µî ´Ù¾çÇÑ ºÎ°¡¼ºñ½ºµéµµ ÃÖ»óÀÇ ¼ºñ½º·Î
Á¦°øÇϰí ÀÖ½À´Ï´Ù.
ÀÚÁÖ
¹®ÀÇÇϽô Ç׸ñµéÀ» Á¤¸®ÇÏ¿´½À´Ï´Ù. [FAQ ¹Ù·Î°¡±â] |
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- 3730xl DNA analyzer
- LIMS (Laboratory Information Management System)¸¦
±â¹ÝÀ¸·Î sample Á¢¼öºÎÅÍ °á°ú ¹ß¼Û±îÁö ½Ç½Ã°£ ¸ð´ÏÅ͸µ
- 24½Ã°£
³»¿¡ °á°ú Å뺸 (¿ÀÈÄ 4½Ã ÀÌÀü ȸ»ç µµÂøºÐ¿¡ ÇÑÇÔ)
- ±âº»ÀûÀÎ ºÐ¼® ¼ºñ½º ¹«·á ´ëÇà (BlastN ¶Ç´Â BlastX¸¦ ÅëÇÑ ´Ü¹éÁú°ú DNA¿°±â¼¿ ¼öÁØ¿¡¼ »óµ¿¼º °Ë»ö)
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Template |
- Plasmid : ³óµµ 100ng/§¡, Volume ±âº» 5ul+(¹ÝÀÀ¼ö*2ul)
- PCR product (purified) : ³óµµ 50ng/§¡, Volume ±âº» 5ul+(¹ÝÀÀ¼ö*2ul)
- PCR product (non-purified) : ³óµµ 100ng/§¡, Volume 20§¡ |
Primer |
- Concentration : 5pmole/§¡ (5pM), Volume : 20§¡
(¿¹)
5 Reaction ¹ÝÀÀ½Ã : 20§¡, reaction ¼ö°¡ Ãß°¡µÉ ¶§¸¶´Ù 2§¡¾¿ Áõ°¡
- °í°´ÀÌ primer Á¦°ø ½Ã ÇÊÈ÷ template¿Í primer ºÐ¸® ¿ä¸Á
- Primer Tm : 55~60¡É, GC content : 40~60%
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Normal plasmid template ±âÁØ, phred score
20 ÀÌ»ó, read length 650 bpÀÌ»ó º¸Àå
- 3730xl ¿Ï·á
- °á°ú ÆÄÀÏ (ab1, txt, pdf, phd) »ý¼º
- BlastN °Ë»ö
- ¹®ÀÚ¸Þ¼¼Áö ÀÚµ¿ ¹ß¼Û
- Data ¹ß¼Û ¹× ¾÷·Îµå
Data Á¢¼ö ¹× process ÁøÇà°úÁ¤¿¡ ´ëÇØ¼ ½Ç½Ã°£ ¸ð´ÏÅ͸µÀÌ °¡´ÉÇÕ´Ï´Ù.
¶ÇÇÑ °í°´´ÔÀÇ ÆíÀǸ¦ À§ÇØ Data ºÐ¼® ¿Ï·á¿Í µ¿½Ã¿¡ ¹®ÀÚ¸Þ¼¼Áö°¡ ¹ß¼ÛµÇ¿À´Ï LIMS ¹× E-mail¿¡¼ °á°úÈ®ÀÎ ¹× download ÇÒ
¼ö ÀÖ½À´Ï´Ù. (±âº»ÀûÀÎ ºÐ¼® ¼ºñ½º)
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Standard-seq single |
°¡Àå ÀϹÝÀûÀÎ single primer extension ¼ºñ½º ÀÔ´Ï´Ù.
Plasmid³ª PCR product¸¦ ÁÖÇüÀ¸·Î ÇÏ°í °í°´´Ô²²¼ ÁöÁ¤ÇϽŠprimer¸¦
ÀÌ¿ëÇØ ±¸°£ÀÇ ¿°±â¼¿ ºÐ¼®À» ¼öÇàÇÏ´Â ¼ºñ½º ÀÔ´Ï´Ù.
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1Primer/1Plate |
" 1 Primer / 1 Plate"´Â ´ÙÀ½ÀÇ µÎ°¡Áö Á¶°ÇÀÌ
¸ðµÎ ¸¸Á·ÇÏ¿©¾ß¸¸ ÇÕ´Ï´Ù.
(1) »ùÇÃÀº ¹Ýµå½Ã 96 or 384 plate¿¡ ´ã°Ü ÀÖ¾î¾ß ÇÕ´Ï´Ù.
(2) °¢ plate´ç ÇѰ³ÀÇ ÇÁ¶óÀ̸Ӹ¸ »ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù.
- »ùÇÃÀ̸§Àº ÀÔ·ÂÇÏ½Ç Çʿ䰡 ¾øÀ¸¸ç, plate À̸§¸¸À» ÀÔ·ÂÇÏ´Â °ÍÀ¸·Î ÃæºÐÇÕ´Ï´Ù.
µû¶ó¼ ½ÃÄö½Ì °á°ú´Â [Plate_name]-[Well_position]_[Primer_name].ab1 ÀÇ Çü½ÄÀ¸·Î ¹ß¼ÛµË´Ï´Ù.
[Plate_name]-[Well_position]_[Primer_name].ab1
ex)
HCVNS5B-H05_SP6.ab1
BRCA2-D24_T7.ab1 |
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2 primers / 1 plate |
" Plate Sequencing"Àº ´ÙÀ½ÀÇ ¼¼°¡Áö Á¶°ÇÀÌ ¸ðµÎ
¸¸Á·ÇÏ¿©¾ß¸¸ ÇÕ´Ï´Ù.
(1) »ùÇÃÀº ¹Ýµå½Ã 96 plate¿¡ ´ã°Ü ÀÖ¾î¾ß ÇÕ´Ï´Ù.
(2) °¢ plate´ç µÎ°³±îÁöÀÇ ÇÁ¶óÀÌ¸Ó »ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù.
(3) ¼¼ °³ ÀÌ»óÀÇ ÇÁ¶óÀ̸Ӹ¦ »ç¿ëÇÏ½Ç °æ¿ì, »ùÇà plate¿Í pair°¡ µÇµµ·Ï primer
plate¸¦ ¸¸µé¾î ÁÖ¼Å¾ß ÇÕ´Ï´Ù.
- »ùÇÃÀ̸§°ú ÇÁ¶óÀÌ¸Ó À̸§À» ¸ðµÎ ÀÔ·ÂÇØ ÁÖ¼Å¾ß Çϸç,
½ÃÄö½Ì °á°ú´Â [sample_name]-[Primer_name].ab1
ÀÇ Çü½ÄÀ¸·Î ¹ß¼ÛµË´Ï´Ù.
[Plate_name]-[Well_position]_[Primer_name].ab1
ex)
HCVNS5B-H05_SP6.ab1
BRCA2-D24_T7.ab1 |
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