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Shotgun Library |
a. Library construction
Genomic shotgun
and large construct shotgun library constructionÀ̶õ
°í°´´ÔÀÇ ¿ä±¸¿¡ µû¶ó ´Ù¾çÇÑ DNA source·Î ºÎÅÍ 1.5~10KbÀÇ ¹üÀ§³»ÀÇ
Insert¸¦ °¡Áö´Â Library¸¦ Á¦ÀÛÇØ µå¸®´Â ¼ºñ½ºÀÔ´Ï´Ù. ±â°£Àº sampleÀÇ
ÀÌ»óÀÌ ¾ø´Â °æ¿ì 4ÁÖ°¡ ¼Ò¿äµË´Ï´Ù.
b. Library construction & Sequencing
Genomic shotgun and large construct shotgun library
constructionÀ̶õ °í°´´ÔÀÇ ¿ä±¸¿¡ µû¶ó ´Ù¾çÇÑ DNA source·Î ºÎÅÍ 1.5~10KbÀÇ ¹üÀ§³»ÀÇ Insert¸¦
°¡Áö´Â Library¸¦ Á¦ÀÛÇϰí, ÀÌ Library¸¦ SequencingÇØ µå¸®´Â ¼ºñ½ºÀ̸ç, ±â°£Àº sampleÀÇ
ÀÌ»óÀÌ ¾ø´Â °æ¿ì 5~6ÁÖ°¡ ¼Ò¿äµË´Ï´Ù. |
TN-Insertion Library |
a. Library construction & Sequencing
TN Insertion library constructionÀ̶õ 8~15kb Á¤µµÀÇ plasmid¸¦
Transposon(Tn) library¸¦ Á¦ÀÛÇÏ¿© ½ÃÄö½ÌÇÏ´Â ¹æ¹ýÀ¸·Î ÀûÀº¾çÀÇ DNA¸¦ »ç¿ëÇÏ¿©, primer
walkingÀ¸·Î ÁøÇàÇÏ´Â ¹æ¹ýº¸´Ù ºü¸£°Ô?sequencingÀ» ÇÏ´Â ¼ºñ½ºÀÔ´Ï´Ù. ±â°£Àº sampleÀÇ ÀÌ»óÀÌ
¾ø´Â °æ¿ì 4ÁÖ°¡ ¼Ò¿äµË´Ï´Ù. |
cDNA Library |
a. Library construction
cDNA library constructionÀ̶õ °í°´´Ô²²¼ ÀÇ·ÚÇϽŠRNA ¶Ç´Â Tissue,
Cell¿¡¼ ºÐ¸®ÇÑ RNA·Î ºÎÅÍ cDNA¸¦ ÇÕ¼ºÇÏ¿© Vector¿¡ LigationÇÏ¿© Library¸¦ Á¦ÀÛÇØ
µå¸®´Â ¼ºñ½ºÀÔ´Ï´Ù. ±â°£Àº sampleÀÇ ÀÌ»óÀÌ ¾ø´Â °æ¿ì 4ÁÖ°¡ ¼Ò¿äµË´Ï´Ù.
b. Library construction & Sequencing
cDNA library constructionÀ̶õ °í°´´Ô²²¼ ÀÇ·ÚÇϽŠRNA ¶Ç´Â
Tissue, Cell¿¡¼ ºÐ¸®ÇÑ RNA·Î ºÎÅÍ cDNA¸¦ ÇÕ¼ºÇÏ¿© Vector¿¡ LigationÇÏ¿© Library¸¦
Á¦ÀÛÇϰí, ÀÌ Library¸¦ SequencingÇØ µå¸®´Â ¼ºñ½ºÀÔ´Ï´Ù. ±â°£Àº sampleÀÇ ÀÌ»óÀÌ ¾ø´Â
°æ¿ì 8ÁÖ°¡ ¼Ò¿äµË´Ï´Ù. |
BAC Library |
a. Library construction
Large insert genomic BAC library constructionÀ̶õ °í°´´ÔÀÇ
¿ä±¸¿¡ µû¶ó ´Ù¾çÇÑ »ý¹°Ã¼·ÎºÎÅÍ HNW (High Molecular Weight) DNA¸¦ ÃßÃâÇÏ¿© 100~200Kb
¹üÀ§³»ÀÇ Insert¸¦ °¡Áö´Â Genomic Library¸¦ Á¦ÀÛÇØ µå¸®´Â ¼ºñ½ºÀÔ´Ï´Ù. ±â°£Àº SampleÀÇ
ÀÌ»óÀÌ ¾ø´Â °æ¿ì 8ÁÖ ÀÌ»ó ¼Ò¿äµË´Ï´Ù.
b. Library construction & Sequencing
Large insert genomic BAC library constructionÀ̶õ °í°´´ÔÀÇ
¿ä±¸¿¡ µû¶ó ´Ù¾çÇÑ »ý¹°Ã¼·ÎºÎÅÍ HNW (High Molecular Weight) DNA¸¦ ÃßÃâÇÏ¿© 100~200Kb
¹üÀ§³»ÀÇ Insert¸¦ °¡Áö´Â Genomic Library¸¦ Á¦ÀÛÇϰí ÀÌ library¸¦ SequencingÇØ
µå¸®´Â ¼ºñ½ºÀÔ´Ï´Ù. ±â°£Àº SampleÀÇ ÀÌ»óÀÌ ¾ø´Â °æ¿ì Library Á¦ÀÛ¿¡ 8ÁÖ ÀÌ»ó ¼Ò¿äµÇ¸ç, SequencingÀº
Sample¾ç¿¡ µû¶ó ¼Ò¿ä±â°£ÀÌ ´Þ¶óÁý´Ï´Ù. |
Cosmid Library |
a. Library construction
Cosmid library constructionÀ̶õ Cosmid Vector¸¦ »ç¿ëÇÏ¿©
DNA Source·ÎºÎÅÍ ÃßÃâÇÑ 40KbÀÇ Insert·Î ±¸¼ºµÈ Library¸¦ Á¦ÀÛÇØ µå¸®´Â ¼ºñ½ºÀÔ´Ï´Ù.
±â°£Àº SampleÀÇ ÀÌ»óÀÌ ¾ø´Â °æ¿ì 4ÁÖ°¡ ¼Ò¿äµË´Ï´Ù.
b. Library construction & Sequencing
Cosmid library constructionÀ̶õ Cosmid Vector¸¦ »ç¿ëÇÏ¿© DNA
Source·ÎºÎÅÍ ÃßÃâÇÑ 40KbÀÇ Insert·Î ±¸¼ºµÈ Library¸¦ Á¦ÀÛÇϰí, ÀÌ Library¸¦ SequencingÇØ
µå¸®´Â ¼ºñ½ºÀÔ´Ï´Ù. ±â°£Àº SampleÀÇ ÀÌ»óÀÌ ¾ø´Â °æ¿ì 5ÁÖ°¡ ¼Ò¿äµË´Ï´Ù. |
Fosmid Library |
a. Library construction
Fosmid library constructionÀ̶õ Fosmid Vector¸¦ »ç¿ëÇÏ¿©
DNA Source·ÎºÎÅÍ ÃßÃâÇÑ 40KbÀÇ Insert·Î ±¸¼ºµÈ Library¸¦ Á¦ÀÛÇØ µå¸®´Â ¼ºñ½ºÀÔ´Ï´Ù.
±â°£Àº SampleÀÇ ÀÌ»óÀÌ ¾ø´Â °æ¿ì 4ÁÖ°¡ ¼Ò¿äµË´Ï´Ù.
b. Library construction & Sequencing
Fosmid library constructionÀ̶õ Fosmid Vector¸¦
»ç¿ëÇÏ¿© DNA Source·ÎºÎÅÍ ÃßÃâÇÑ 40KbÀÇ Insert·Î ±¸¼ºµÈ Library¸¦ Á¦ÀÛÇϰí, ÀÌ Library¸¦
SequencingÇØ µå¸®´Â ¼ºñ½ºÀÔ´Ï´Ù. ±â°£Àº SampleÀÇ ÀÌ»óÀÌ ¾ø´Â °æ¿ì 5ÁÖ°¡ ¼Ò¿äµË´Ï´Ù.
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