The purpose of the Terms is to establish the rights and obligations of Macrogen and Client with respect to Macrogen's service or product as requested or ordered by Client. Macrogen and Client shall faithfully perform their duties as specified in these Terms.
Unless specified otherwise in these Terms, the following terms have the meanings set forth herein.
These Terms shall become effective as of the date when Client places an order and shall remain in full force and effect till the completion date unless agreed otherwise.
| Service name | Analysis sample | Data |
|---|---|---|
| CES | 90 calendar days | 3 years |
| Nanopore SEQ | 30 calendar days | 30 calendar days |
| Oligo | N/A | 3 years |
| NGS | 30 calendar days | 30 calendar days |
| Clinical | 90 calendar days | 90 calendar days |
| Chip | 90 calendar days | 90 calendar days |
After placing an order, Client may request and receive additional technical support or consulting service from Macrogen. In the event that such support or service incur any additional costs, Macrogen shall notify and discuss with the client in advance.
본 약관의 적용을 위한 용어는 다른 특별한 언급이 없는 한 다음 각 호의 의미로 사용된다.
본 약관의 유효기간은 별도로 양 당사자의 서면합의에 의한 변경이 없는 한 주문 등록일로부터 서비스 또는 제품 제공과 대금지급을 포함한 본 약관에 따른 모든 이행사항이 완료되는 시점까지로 한다.
주문자 또는 마크로젠이 본 약관에서 정한 사항을 위반하여 상대방에게 손해를 입힌 경우에는 귀책사유가 있는 당사자는 상대방의 손해를 배상하여야 한다. 다만, 주문자에게 부담하는 마크로젠의 손해배상 범위는 관련 주문건에 해당하는 대금의 총액을 초과할 수 없다.
주문 후 주문자는 마크로젠에 추가적인 기술 지원 또는 자문을 요청 할 수 있으며, 이에 따른 별도 비용이 발생하는 경우에는 마크로젠은 사전에 이를 주문자에게 안내하고 주문자와 관련사항을 협의하여야 한다.
각 당사자는 상대방의 사전 서면동의 없이 본 약관상의 지위, 권리 또는 의무의 전부 또는 일부를 임의로 제 3자에게 양도, 담보제공 또는 대여 등 일체의 처분행위를 할 수 없다.
Como usar Pick-Up servicio con GRATIS en Santiago?
How to use Pick-Up service for free in Santiago?
Tienes pregunta?
Contacto +56 9 5845 1395
Envianos tus muestras de forma segura a Chile
The safest way to deliver your sample to Chile?
Tienes pregunta?
Contacto +56 9 5845 1395
Whole Plasmid Sequencing (WPS) is a long-read sequencing service powered by Oxford Nanopore Technologies (ONT). It enables comprehensive plasmid sequence characterization through de novo assembly, without sequencing primer design or primer walking. Analyze both insert and vector backbone regions in a streamlined workflow.
Sanger sequencing can be suitable for targeted verification of a specific nucleotide change in a defined region. WPS is designed for comprehensive verification of the plasmid, including inserts and backbone regions. If you need precise base-level confirmation of a point mutation, Sanger sequencing is recommended.
| Category | Requirements |
|---|---|
| Sample Type | Purified plasmid DNA (single plasmid) |
| DNA Concentration | 50 ~100 ng/µL |
| Volume | ≥ 15 µL |
| Quantification | Fluorometric quantification methods are recommended |
| Purity | A260/280: 1.8–2.0 (measured by NanoDrop) |
| Plasmid Size | 1–300 kb |
| Buffer | Nuclease-free Water or TE Buffer |
| Submission format | PCR Tube(Strip) or 96-well Plate recommended |
We provide the shipping protocol to make all users of Macrogen’s life easier!
Air waybills and commercial invoices will be provided to you in a few clicks through this protocol.
As your account carries all of your shipping information, sender information will be automatically submitted to you.
You can also save up to three different addresses for your future orders.
You are able to print the waybill, the commercial invoice and the letter of acceptance with all shipping details already entered according to the waybill information when you click the Continue button.
When all three steps have been completed, call your local FedEx or DHL to request for pickup!
Don’t worried about the safe arrival of your samples after dispatching,
we have the monitoring system for the packages that you send us to promptly resolve all the problems happened during shipping.
Report
Result
Sequencing Report (.html) — Sample status
A comprehensive analysis report providing the Plasmid Map, sequence annotations, assembly results, and read quality statistics. 'Length' indicates the consensus sequence length (bp), and 'Mean Quality' represents the Q-score.
HTML report (.HTML)
Interactive clone validation analysis, including assembly, annotations, read statistics and self-alignment dot plots
Plasmid map
Visualize functional elements such as predicted genes, CDS, promoters, and markers on a circular map based on the assembled sequence. Annotations are predicted based on databases.
Chromatogram
A synthetic chromatogram generated from the consensus sequence, where peak height represents the frequency of bases from aligned reads at each position.
Note: The provided Consensus AB1 file is generated solely from sequence information and differs from the chromatogram shown in the report.
Virtual gel
An in silico electrophoresis image created from the read length distribution. Band position indicates read length, and band intensity represents the frequency of reads at that length.
Read statistics
Examine the quality and distribution of sequencing data, including read quality, read length, and base yield.
Dot plot
A visual representation to identify features such as repeat regions and reverse-complementary sequences within the assembled sequence.
Consensus FASTA (final.fasta)
Final assembled plasmid consensus sequence
Consensus FASTQ (final.fastq)
Final assembled consensus sequence with base quality scores
Note: Raw FASTQ files (.fastq.gz) contain all individual reads and are distinct from the final assembled consensus FASTQ file. Please use consensus files to verify your plasmid sequence.
Consensus AB1 (.ab1)
Assembled consensus sequence provided in AB1 format, which can be viewed using standard chromatogram viewers
Note: Generated solely from sequence information without read-specific base frequencies and differs from actual Sanger chromatogram data.
GenBank (annotations.gbk)
Assembled sequence with available functional annotations
Raw FASTQ (.fastq.gz)
Raw sequencing read data generated from the long-read sequencing platform
Note: Raw FASTQ files (.fastq.gz) contain all individual reads and are distinct from the final assembled consensus FASTQ file. Please use consensus files to verify your plasmid sequence.
※ The contents of the report and annotations may vary depending on the actual analysis results.
| Item | Notice |
|---|---|
| Viewing Consensus AB1 files | The Consensus AB1 file can be viewed using programs such as SnapGene, Chromas, UGENE, and FinchTV. Please note that this file provides the consensus sequence in AB1 format and differs from actual chromatogram data generated by Sanger sequencing. |
| Result downloads | Result files are available for download for 14 days from the date of result delivery. Please ensure you download and save your files separately within this period. |
| Data retention & deletion | Download links for result files are active for 14 days (2 weeks) from the delivery date. Raw sequencing data and analysis results are permanently deleted after 30 days, and deleted data cannot be recovered. |
| Sample retention period | Samples are discarded 30 days after the result delivery date. However, if 'Immediate Disposal' is selected during ordering, samples will be discarded within 72 hours of result delivery. |
| Issue | Possible Causes | Recommended Action |
|---|---|---|
| Low DNA Concentration | DNA concentration or total amount falls below the recommended criteria. | Accurately measure the DNA concentration and ensure the sample meets the minimum requirements of 50 ~100 ng/µL and at least 15 µL before submission. |
| Low DNA Purity | Residual purification reagents (e.g., guanidine salts, phenol, ethanol) inhibiting library preparation enzymes. | Check the A260/230 ratio alongside the A260/280 ratio. Pure DNA typically has an A260/230 ratio of 2.0–2.2. A lower value indicates potential reagent carryover. Please re-purify the sample before submitting. |
| DNA Degradation | DNA fragmentation due to repeated freeze-thaw cycles or exposure to high temperatures. | Review your storage and shipping conditions. Avoid repeated freeze-thaw cycles and exposure to inappropriate temperatures. |
| Mixed Plasmid / Genomic DNA Contamination | Presence of multiple different plasmids, or host (e.g., E. coli) genomic DNA extracted during plasmid purification. Excessive vortexing can lead to host DNA contamination. | Ensure the purified DNA is derived from a single clonal source. Review your internal cell culture and purification steps to minimize host genomic DNA contamination by avoiding excessive vortexing. Mixed or contaminated samples can lead to reduced effective reads, lower coverage, poor assembly, or analysis failure. |
| Issue | Possible Causes | Recommended Action |
|---|---|---|
| Low Sequencing Depth | Poor DNA quality, low library preparation efficiency, or reduced sequencing yield. | Verify the concentration, purity, and integrity of your sample. Final coverage may vary depending on sample characteristics and analysis conditions. |
| Incomplete Assembly | Insufficient effective reads, repetitive sequences, or mixed sample sequences preventing successful assembly. | Confirm the sample quality and ensure it contains only a single plasmid construct. |
| Unexpected Assembly Size | Formation of plasmid dimers/multimers, repetitive sequences, or other assembly-related factors. | If the assembled sequence differs from your expected plasmid size, please contact us with your order details for further review. |
| Unexpected Plasmid Sequence | A mixture of different plasmids in the submitted sample, or submission of an unintended clone. | Review your internal clone selection and sample preparation workflows, and examine the analysis results alongside your expected sample information. |
| Homopolymer / Short Tandem Repeat | Insertion or deletion (indel) errors in homopolymer regions (e.g., AAAAAA) or short tandem repeats due to the characteristics of nanopore sequencing. | Base-level accuracy cannot be fully guaranteed in these specific regions. If precise verification is required, we recommend using Sanger sequencing as a complementary method. |
* Free shipping is provided for orders of 10 or more samples
| Service | Plasmid Size | Type | TAT | Price |
|---|---|---|---|---|
| Whole Plasmid Sequencing | 1–5 kb | Sample | 1~3 Business Days | Request a Quote |
| 5–25 kb | Sample | 1~3 Business Days | ||
| 25–125 kb | Sample | 1~3 Business Days | ||
| 125–300 kb | Sample | 1~3 Business Days |
This agreement is a contract between you and Macrogen Inc. and applies to Macrogen’s services usage in whole. You shall read, agree with and accept all of the terms and conditions contained in this agreement.
This agreement is to comply with the law of electric communication enterprise and an Enforcement Ordinance in Korea on the utilization stipulation and procedure of all the related service provided by Macrogen Inc.
Service defines that it furnishes DNA sequencing and other additional information through http://dna.macrogen.com to be provided by Macrogen Inc hereunder.
Macrogen can delete the notice posted by users for the following without any pre-notice.
All disputes, controversies or differences which may arise out of or in connection with the Contract or for the breach thereof shall be finally settled by arbitration in Seoul, Korea in accordance with the Commercial Arbitration Rules of the Korean Commercial Arbitration Board and the laws of Korea.
The Contract shall be governed and interpreted by the laws of the Republic of Korea.
Unless otherwise provided herein, Supplier shall not be liable for any consequential, direct or indirect damages, related to free service , except for the damages caused by willful misconduct.
Macrogen lifetime storage service will be provided to all of our customers at free of charge up to fifteen (15) years from the date of data creation.
We are not liable for damage or losses to any of one’s standard sequencing result files which are stored in Macrogen’s server resulting from participation in or accessing or downloading file or data in connection with the Service.
We reserve the right, in our sole discretion, to cancel or suspend the Service should a virus, bugs, or other causes beyond our control corrupt the administration, security or proper operation of the Service.
You shall pay liquidated damages, not as a penalty, to Macrogen in an amount of 10% of total amount of the delayed payment behind due date.