Capillary Electrophoresis Sequencing (CES) is a service that analyzes DNA sequences using a biochemical method.
The CES service of Macrogen maximizes customer satisfaction with various service lineups such as identification services, which involves the differentiation and identification of species of living organisms, fragment analysis services, and customized sequencing services newly applied from the existing sequencing method, as well as standard sequencing that is the most common service.
CES is used in a wide range of fields such as a basic research in molecular biology, breeding research, genetic disease research, paternity confirmation and forensic research, and has become a key in the continued development of life sciences.
Separate online order system is also available for customers’ convenience so that they can request a desired service.
Monitoring of all processes and follow-up for the results are provided for each ordered sample.
Standard sequencing is a service that sequences PCR products and plasmid DNA requested by customers.
Macrogen provides quicker and more accurate services based on the Capillary Electrophoresis Sequencing (CES)
automation system and extensive experience.
After the results are delivered, an expert in the sequencing field is ready to provide help until the follow-up.
Standard sequencing can be performed with single tubes and 96-well plates.
In PCR product and Plasmid samples, it may be difficult to verify the sequence result as the basic experimental condition.
This service is designed to check for improved sequencing results using non-normal structures and appropriate reagents and conditions.
Primer walking is a service that analyzes the sequence of plasmids or PCR products that cannot be read at once by single primer extension.
It is generally used to obtain about 2 to 10kb of sequence information.
End sequencing is performed with a primer provided or specified by the customer, and the internal primer is designed and produced based on the results.
This is extended continuously by redesigning the internal primer in a suitable location from the results obtained
by the new primer’s reaction with the same template. Walking takes about 4 days, and can be extended about 500 ‒ 800 bp in one direction.
EZ-seq brings easiness and simplicity to your daily DNA sequencing process. EZ-seq is a prepaid barcode service,
in which you order barcode online, receive them by courier service and use them for sequencing.
EZ-seq reduces the workload of placing orders and also the number of invoices as you will get one invoice for all barcodes.
All you need to do is to mix the template with the sequencing primer and ship the samples to our laboratory with the EZ-seq barcode labels attached.
We have been dedicating to provide genomic solutions to researchers in all over the world with its advanced DNA sequencing technology by using ABI 3730XLs. Thanks to over 20 years long know-how and large genomics facility, we are able to provide our sequencing service from small scale sequencing reactions to large sequencing projects, we are ready to serve you with our pleasure.
Send us your gDNA and primers. After checking your sample quality, our R&D specialist team will proceed with
PCR amplification, PCR product purification and sequencing with the most optimum condition.
If you select a gene part of your choice and send us a sample, we will provide you with the entire testing process
from genomic DNA extraction to sequence analysis.
We provide services to analyze genetic differences in Genome, such as SNP and Mutation,
through base sequence analysis using Macrogen’s Sanger sequencing method.
One-click Sanger sequencing is a method of conveniently and quickly analyzing certain commonly studied
gene variations with just one click. Optimized results from gDNA extraction to PCR amplification,
sequencing and SNP analysis are delivered to customers.
Macrogen’s expert researchers perform sequencing of specific exon areas
and provide variant analysis results using a primer set manufactured based on many years of know-how.
NGS Next Generation Sequencing (NGS) validation is a service that verifies data using Sanger sequencing
to improve the accuracy and reliability of data after using the NGS service.
When the customer specifies the chromosome position or reference sequence,
we analyze SNP of a certain area and the mutation results..
PCR optimization is a customized service that performs and analyzes the entire process from primer design to PCR amplification,
sequencing and BI report when the customer provides references together with cell/gDNA in relation to the desired target region.
If the retest rate is high due to the rarity of the sample in PCR amplification, touchdown PCR amplification,
nested PCR amplification and FFPE PCR amplification, Macrogen solves the problem by using its customized PCR amplification service.
16S/18S/26S rRNA and ITS region full sequencing is a service that analyzes sequences
by amplifying ribosomal RNA genes of bacteria and fungi (filamentous fungus and yeast)
and confirming homology of target microorganisms using an rRNA database (NCBI).
Macrogen controls and performs all processes from gDNA extraction of bacteria
and fungi (filamentous fungus and yeast) to PCR amplification, purification, sequencing, and BI report.
For bacteria, PCR of 16S rRNA genes is performed using 27F and 1492R primers, and sequencing is conducted using 785F
and 907R primers, which are the inter-primers, to identify bacteria. If the customer requests,
a primer can be additionally selected/changed and about 1,350 bp or longer sequences can be provided.
The default 2rxn (785F, 907R) is selected to allow more primer runs if desired.
1,600 bp or higher results are guaranteed by the sequencing of the 18S rRNA region.
500 bp or longer results are guaranteed by the sequencing of ITS region.
1,300bp or longer results are guaranteed by the sequencing of 26S rRNA gene (D1/D2/D3 region).
Microbial mass analysis and identification service using MALDI-TOF protein analysis technology uses
the world's first FDA Clearance acquisition and AOAC certified system.
This service is suitable for customers who want accurate microbial coordination
while taking shorter time than the existing basic sequence compassion service.
To classify subspecies within the same species, such as Bacteria and Fungi,
the PCR amplification is conducted using 6 to 8 housekeeping genes that are homogenous.
The association between sequence types (STs) can be analyzed through data assemble by sequencing 450 to 600bp internal structure
to reveal genetic and molecular evolutionary relationships through a combination of various antagonists of different subspecies
within the same species.
The fragment analysis service includes various services such as genotyping, DNA profiling,
medical mutation detection and agricultural research.
Macrogen provides a microsatellite analysis (VNTRs) service based on
our extensive experience and expertise.
Fragment analysis is a service that separates and analyzes amplified PCR products according
to fragments using a primer marked by a fluorescent label.
(Maximum detection size)
|350 ROX (350bp)||120 LIZ (120bp)|
myPETGENE provides genetic testing services for companion animals. The genetic testing service of myPETGENE can predict their genetic diseases early and prevent them with customized control, such as improvement in eating habits and exercise. DNA can be used to identify the individual animal and prove blood ties, preventing their loss and abandonment and helping the breeding of healthy companion animals.
In some countries, it is already common to obtain the identification of an individual animal and certification for
genetic diseases with genetic testing and a culture of breeding healthy animals is established.
myPETGENE regards your companion animals as precious family members. We promise to provide more accurate and superior genetic testing services to seek a healthier life for companion animalsand establish a proper culture for companion animals.
Human ID provides a personal gene identification service upon research.
Human ID offers a paternity test that can confirm lineal ascendant relationships with a comparative analysis of the unique DNA patterns of individuals, a maternity test that can prove the maternal blood relationships of a female, DNA analysis of human remains for the identification of a person killed in a war, and personal identification testing.
Human ID can perform gene analysis tests with various specimens that can be tested such as hair, epithelium cells, body remains, and saliva, providing reliable services with 99.99% accurate test results.
Animal/Plant Identification is a service that identifies a species of living organism by sequencing “DNA Barcode,”
which is a region used for the identification of species in the gene of a living organism.
If you send us a sample, then we will process it from the extraction of gDNA to the sequencing result of the barcode area and provide the analysis report.
|Division||Sample Type||Genes to Analyze||Additional Analysis|
|Animal Barcoding||Animals, Insects, Birds, Fish, Etc||COI||12S/16S rRNA, ITS|
|Plant Barcoding||Plants||matK, rbcL||rpoB, rpoC1, atpF-atpH, psbK-psbI. psbA-trnH|
Pyrosequencing is a sequencing service using pyrophosphate (PPi) generated when the polymerization of nucleotide with DNA occurs. Since there are more epigenetics studies on controlling the expression level of certain genes regardless of DNA sequence,
the DNA methylation analysis service is gaining more attention.
For DNA methylation analysis, very accurate and reliable results can be expected by the pyrosequencing service.
This service provides quantitative analysis of the low frequency alle and the thermal motion of the target position in gDNA.
This service quantitatively analyzes the methylation of the target CpG position after processing gDNA bisulfate.
|Template / Service||Concentration||Sample Volume||Primer Volume
|PCR product (below 600bp)
Purified / Unpurified
|5-10ng /ul per 100bp|
|PCR product (over 600bp)
Purified / Unpurified
|5-10ng /ul per 100bp|
|Primer Walking||100~150ng/ul||10~15ul per 1kb||1-1.5ug / kb
(for an insert size of up to 4kb)
|16S/18S/26S rRNA & ITS region
full Sequencing (gDNA)
|30~50ng/ul||50ul||* Agar Plates: Petri dishes
* Glycerol Stocks: 1.5ml tubes.
|PCR amplification (gDNA)||> 50ng||10ul
We provide the shipping protocol to make all users of Macrogen’s life easier!
Air waybills and commercial invoices will be provided to you in a few clicks through this protocol.
As your account carries all of your shipping information, sender information will be automatically submitted to you.
You can also save up to three different addresses for your future orders.
You are able to print the waybill, the commercial invoice and the letter of acceptance with all shipping details already entered according to the waybill information when you click the Continue button.
When all three steps have been completed, call your local FedEx or DHL to request for pickup!
Don’t worried about the safe arrival of your samples after dispatching,
we have the monitoring system for the packages that you send us to promptly resolve all the problems happened during shipping.
The purpose of the re-sequencing of the Macrogen Sequencing Service is to verify the machine’s error or non-treatable operability,
and only once for those things that are deemed to be possible for improvement.
* The primer binding site might be damaged. We would like to recommend that you try to use an alternative primer.
This agreement is a contract between you and Macrogen Inc. and applies to Macrogen’s services usage in whole. You shall read, agree with and accept all of the terms and conditions contained in this agreement.
This agreement is to comply with the law of electric communication enterprise and an Enforcement Ordinance in Korea on the utilization stipulation and procedure of all the related service provided by Macrogen Inc.
Service defines that it furnishes DNA sequencing and other additional information through http://dna.macrogen.com to be provided by Macrogen Inc hereunder.
Macrogen can delete the notice posted by users for the following without any pre-notice.
All disputes, controversies or differences which may arise out of or in connection with the Contract or for the breach thereof shall be finally settled by arbitration in Seoul, Korea in accordance with the Commercial Arbitration Rules of the Korean Commercial Arbitration Board and the laws of Korea.
The Contract shall be governed and interpreted by the laws of the Republic of Korea.
Unless otherwise provided herein, Supplier shall not be liable for any consequential, direct or indirect damages, related to free service , except for the damages caused by willful misconduct.
Macrogen lifetime storage service will be provided to all of our customers at free of charge up to fifteen (15) years from the date of data creation.
We are not liable for damage or losses to any of one’s standard sequencing result files which are stored in Macrogen’s server resulting from participation in or accessing or downloading file or data in connection with the Service.
We reserve the right, in our sole discretion, to cancel or suspend the Service should a virus, bugs, or other causes beyond our control corrupt the administration, security or proper operation of the Service.
You shall pay liquidated damages, not as a penalty, to Macrogen in an amount of 10% of total amount of the delayed payment behind due date.