NGS(Next Generation Sequencing) is the method of reading a genome by dividing it into
many pieces, assembling the obtained sequence pieces and analyzing the
sequence of the whole genome.
Macrogen produces a large amount of genome information every year using various kinds of
equipment, provides quicker and more accurate genome analysis service at an economical
price to help studies conducted by researchers, and offers a diagnostic service to the public.
We provide genome analysis services suitable for research, such as whole genome, exome,
transcriptome, epigenome and metagenome, and bioinformatics results based on the data.
Whole Genome Sequencing (WGS) is a method of reading the entire genome
and analyzing related genetic information.
· Whole Genome De novo Sequencing
· Whole Genome Resequencing
As a method that newly identifies the genome information of new species with no reference information,
De novo Sequencing offers a way to uncover information about the entire genome of microorganisms or animals and plants that are not known.
Resequencing is a method that allows variation analysis, such as Single Nucleotide Polymorphism (SNP), Insertion
and Deletion (InDel), Copy Number Variation (CNV), and structural variation analysis, using already-known reference genomes.
The variation information obtained by resequencing is used to discover genes related to diseases and to conduct personalized medicine.
Metagenome sequencing is a method of identifying microbial communities
that exist in different environments. It is used primarily to analyze the distribution
and types of bacteria and fungi.
In other words, it is used to determine which microbial communities are present
in a sample collected in a certain environment, and to identify their interactions and roles.
Moreover, the fusion primer is produced and experiments are conducted by mixing various samples,
thus an analysis on archaea as well as bacteria can be performed.
The number of genes in intestinal microorganisms is about 150 times greater than human genes.
In addition, it is applied to various areas close to humans, such as monitoring microbial clusters
through environmental samples such as soil and sea water, and analysis of fermented food,
environmental wastewater, and lactobacillus.
Targeted Sequencing is a method of selective analysis on the areas researchers are interested in.
· Customized Targeted Sequencing
For PCR Optimization service, please provide Cell/gDNA with reference in relation to the target reference.
This service is customized for customers who perform and analyze the entire process process from primer design to PCR amplification,
sequencing, and BI report.
In addition, if the re-test rate is high due to sample specificity, such as PCR amplification, Touch down PCR amplification,
Nested PCR amplification, FFPE PCR amplification, etc.
Customer-tailored PCR amplification service helps you solve the problem.
Exome sequencing is a method of selective analysis in an exon area known to be where genes exist.
It is a more effective and economical way of performing research than whole genome resequencing
because it selectively analyzes only the genes obtained from current studies.
Whole exon parts can be captured and variation analyses such as SNP and InDel are provided.
Transcriptome sequencing, which targets RNA, is one of the most commonly used research methods.
When studying a living organism with no reference genome information, analysis of the transcriptome
of the living organism is possible by de novo assembly.
The difference in gene expression values (expression profiling) can be verified
by transcriptome analysis.
· mRNA/mRNA + lncRNA Sequencing
· Single Cell RNA Sequencing
· Small RNA Sequencing
· Extracelluar RNA/ Exosomal RNA Sequencing
mRNA sequencing is more accurate because it indicates expression by accumulating the numbers of sequence that refer to a gene. It can
be used in various studies because information on novel transcriptome, fusion gene, and alternative splicing variant can also be verified.
If traditional transcriptome sequencing is the way that organizations see the average gene expression in different cells,
then Signal Cell RNA Sequencing can analyze thousands of transcription bodies simultaneously at a single cell level,
so we can see the difference in gene expression values for different cell types.
In addition, a full-length (clonotype level) analysis of the B cell, T cell is possible.
Small RNA that contains miRNA and siRNA is the RNA-based key factor of gene expression control mechanism that is conserved in
mammals. It is known to be involved in the control of gene expression in the generation process.
Studies are actively being conducted especially in the mechanism of non-encrypted small RNA that is of 21-23 bases acting
as siRNA or miRNA.
Extraccellular RNA or exosomal RNA, which exists outside cells in living fluids such as plasma or urine, can be used in various studies such as
liquid biopsy through analysis of various transcription bodies (miRNA, piRNA, snRNA, snRNA, snRNA, and mRNA).
From Exosome separation to small RNA profiling and comparative analysis are possible.
By creating a full-length transcript, you can characterize the transcript isoform across the Target gene or Transcriptome.
Specifically, it provides accurate information about the Alternately Splitted Exon and Transcriptive Start Site (TSS).
Epigenome sequencing confirms that the function of a certain gene is expressed by external environmental factors.
Methylation of DNA and acetylation of histone protein are the most representative epigenetic control mechanisms
for the transcription of genes. They can be analyzed more effectively with NGS technology.
· Whole Genome Bisulfite Sequencing
· Methyl-capture Sequencing
WGBS is a method of observing the degree of DNA methylation by treating DNA using sodium bisulfate,
which converts non-methylated cytosine to uracil, and analyzing the sequence.
Methyl-Capture Sequencing is a method that captures the parts of the CpG where methylation was
previously reported and analyzes the sequence converted to sodium sulfite to observe the methylation degree.
|Methyl-capture Sequencing||Yes||Methylated CpG enrichment|
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