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NGS(Next Generation Sequencing) is the method of reading a genome by dividing it into
many pieces, assembling the obtained sequence pieces and analyzing the
sequence of the whole genome.

Macrogen produces a large amount of genome information every year using various kinds of
equipment, provides quicker and more accurate genome analysis service at an economical
price to help studies conducted by researchers, and offers a diagnostic service to the public.

We provide genome analysis services suitable for research, such as whole genome, exome,
transcriptome, epigenome and metagenome, and bioinformatics results based on the data.

Whole Genome Sequencing

Whole Genome Sequencing (WGS) is a method of reading the entire genome
and analyzing related genetic information.

· Whole Genome De novo Sequencing
· Whole Genome Resequencing

Whole Genome De novo Sequencing

As a method that newly identifies the genome information of new species with no reference information,
De novo Sequencing offers a way to uncover information about the entire genome of microorganisms or animals and plants that are not known.

Sequencing Platform
· HiSeq 2500 / HiSeq 4000 / NovaSeq 6000
· NextSeq 500 / MiSeq
· PacBio RSII / Sequel
Whole Genome Resequencing

Resequencing is a method that allows variation analysis, such as Single Nucleotide Polymorphism (SNP), Insertion
and Deletion (InDel), Copy Number Variation (CNV), and structural variation analysis, using already-known reference genomes.
The variation information obtained by resequencing is used to discover genes related to diseases and to conduct personalized medicine.

Sequencing Platform
· HiSeq 2500 / HiSeq 4000 / HiSeq X Ten / NovaSeq 6000
· NextSeq 500

Metagenome Sequencing

Metagenome sequencing is a method of identifying microbial communities
that exist in different environments. It is used primarily to analyze the distribution
and types of bacteria and fungi.
In other words, it is used to determine which microbial communities are present
in a sample collected in a certain environment, and to identify their interactions and roles.

Moreover, the fusion primer is produced and experiments are conducted by mixing various samples,
thus an analysis on archaea as well as bacteria can be performed.
The number of genes in intestinal microorganisms is about 150 times greater than human genes.


In addition, it is applied to various areas close to humans, such as monitoring microbial clusters
through environmental samples such as soil and sea water, and analysis of fermented food,
environmental wastewater, and lactobacillus.

Sequencing Types & Platforms
  • · Meta Amplicon Sequencing : MiSeq / HiSeq2500
    - 16S rRNA V3-4 region
    - ITS2 region
    -  Customized region : 18S rRNA, CO1, etc
  • ·Meta Shotgun Sequencing : HiSeqX / NovaSeq
  • ·Full length 16S rRNA : PacBio RSII / Sequel

Targeted Sequencing

Targeted Sequencing is a method of selective analysis on the areas researchers are interested in.

· Customized Targeted Sequencing

PCR Optimization

For PCR Optimization service, please provide Cell/gDNA with reference in relation to the target reference.
This service is customized for customers who perform and analyze the entire process process from primer design to PCR amplification,
sequencing, and BI report.

In addition, if the re-test rate is high due to sample specificity, such as PCR amplification, Touch down PCR amplification,
Nested PCR amplification, FFPE PCR amplification, etc.
Customer-tailored PCR amplification service helps you solve the problem.

Exome Sequencing

Exome sequencing is a method of selective analysis in an exon area known to be where genes exist.
It is a more effective and economical way of performing research than whole genome resequencing
because it selectively analyzes only the genes obtained from current studies.
Whole exon parts can be captured and variation analyses such as SNP and InDel are provided.

Main Capture kit
· Agilent SureSelect Exome Capture kit
· Truseq Exome Enrichment Kit
· Twist Human Core Exome Kit
Sequencing Platforms
· HiSeq 2500 / HiSeq 4000 / HiSeq X Ten / NovaSeq 6000
· NextSeq 500

Transcriptome Sequencing

Transcriptome sequencing, which targets RNA, is one of the most commonly used research methods.
When studying a living organism with no reference genome information, analysis of the transcriptome
of the living organism is possible by de novo assembly.

The difference in gene expression values (expression profiling) can be verified
by transcriptome analysis.

· mRNA/mRNA + lncRNA Sequencing
· Single Cell RNA Sequencing
· Small RNA Sequencing
· Extracelluar RNA/ Exosomal RNA Sequencing
· Iso-seq

mRNA/mRNA+lncRNA Sequencing

mRNA sequencing is more accurate because it indicates expression by accumulating the numbers of sequence that refer to a gene. It can
be used in various studies because information on novel transcriptome, fusion gene, and alternative splicing variant can also be verified.

Library Construction Kit
· TruSeq RNA Access
· TruSeq Stranded Total RNA with Ribo-Zero H/M/R
· TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold
· TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Globin
· TruSeq Stranded Total RNA with Ribo-Zero Plant
· TruSeq mRNA
· TruSeq mRNA(Insert size)
· TruSeq stranded mRNA
· TruSeq Total RNA with Ribo-Zero
· TruSeq Total RNA with Ribo-Zero(Insert size)
· TruSeq Stranded Total RNA (NEB Microbe)

· TruSeq Total RNA (NEB Microbe)

· SMARTer Universal Low RNA library
· SMARTer Ultra Low Input RNA library
· SMARTer Stranded RNA library
· SureSelect RNA Direct_Human
· SureSelect RNA Direct_Mouse
Sequencing Platforms
· HiSeq 2500 / HiSeq 4000 / NovaSeq 6000
· NextSeq 500
Single Cell RNA Sequencing

If traditional transcriptome sequencing is the way that organizations see the average gene expression in different cells,
then Signal Cell RNA Sequencing can analyze thousands of transcription bodies simultaneously at a single cell level,
so we can see the difference in gene expression values for different cell types.

In addition, a full-length (clonotype level) analysis of the B cell, T cell is possible.

Library  construction Kit
· 10x chromium 5'GEX, 
· 10x chromium 3'GEX
· 10x chromium V(D)J
Sequencing Platforms
· HiSeq 2500 / HiSeq 4000 / NovaSeq 6000
Small RNA Sequencing

Small RNA that contains miRNA and siRNA is the RNA-based key factor of gene expression control mechanism that is conserved in
mammals. It is known to be involved in the control of gene expression in the generation process.

Studies are actively being conducted especially in the mechanism of non-encrypted small RNA that is of 21-23 bases acting
as siRNA or miRNA.

Library Construction Kit
· Small RNA library

· SMARTer smRNA library
· NEBNext Small RNA library
Sequencing Platforms
· HiSeq 2500 / HiSeq 4000
· NextSeq 500
Extracelluar RNA/ Exosomal RNA Sequencing

Extraccellular RNA or exosomal RNA, which exists outside cells in living fluids such as plasma or urine, can be used in various studies such as
liquid biopsy through analysis of various transcription bodies (miRNA, piRNA, snRNA, snRNA, snRNA, and mRNA).
From Exosome separation to small RNA profiling and comparative analysis are possible.

Library Construction Kit
· Small RNA library

· SMARTer smRNA library
· NEBNext Small RNA library
Sequencing Platforms
· HiSeq 2500 / HiSeq 4000
· NextSeq 500

By creating a full-length transcript, you can characterize the transcript isoform across the Target gene or Transcriptome.
Specifically, it provides accurate information about the Alternately Splitted Exon and Transcriptive Start Site (TSS).

Library Construction Kit
· PacBio Sequel Iso-Seq library construction
Sequencing Platforms
· Sequel

Epigenome Sequencing

Epigenome sequencing confirms that the function of a certain gene is expressed by external environmental factors.

Methylation of DNA and acetylation of histone protein are the most representative epigenetic control mechanisms
for the transcription of genes. They can be analyzed more effectively with NGS technology.

· Whole Genome Bisulfite Sequencing
· Methyl-capture Sequencing

Whole Genome Bisulfite Sequencing(WGBS)

WGBS is a method of observing the degree of DNA methylation by treating DNA using sodium bisulfate,
which converts non-methylated cytosine to uracil, and analyzing the sequence.

Sequencing Platform
· HiSeq2500/ HiSeq4000/ HiSeq X Ten / NovaSeq6000
Methyl-Capture Sequencing

Methyl-Capture Sequencing is a method that captures the parts of the CpG where methylation was
previously reported and analyzes the sequence converted to sodium sulfite to observe the methylation degree.

Comparison of Epigenomic Application
Application Bisulfite Conversion Advantages
WGBS Yes High resolution
Methyl-capture Sequencing Yes Methylated CpG enrichment
Sequencing Platform
· HiSeq2500/ HiSeq4000/ HiSeq X Ten/ NovaSeq6000
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'(주)마크로젠'는 (이하 '회사'는) 정보통신망 이용촉진 및 정보보호 등에 관한 법률, 개인정보보호법, 통신비밀보호법, 전기통신사업법, 등 정보통신서비스 제공자가 준수하여야 할 관련 법령상의 개인정보보호 규정을 준수하며, 회사는 개인정보처리방침을 통하여 고객님께서 제공하시는 개인 정보가 어떠한 용도와 방식으로 이용되고 있으며, 개인정보보호를 위해 어떠한 조치가 취해지고 있는지 알려드립니다.

회사는 개인정보처리방침을 개정하는 경우 웹사이트 공지사항(또는 개별공지)을 통하여 공지할 것 입니다.

1.이용하는 개인정보 항목

- 이름, 이메일 주소, 기관 명

2. 개인정보의 이용목적

회사는 수집한 개인정보를 다음의 목적을 위해 활용합니다.

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- 이용 목적 달성 시 까지

※ 위와 같은 개인정보 수집 이용에 대하여 동의를 거부할 권리가 있습니다. 그러나 동의를 거부할 경우 고객 문의 시 서비스가 제한될 수 있습니다.

Privacy Policy


General Terms & Conditions

This agreement is a contract between you and Macrogen Inc. and applies to Macrogen’s services usage in whole. You shall read, agree with and accept all of the terms and conditions contained in this agreement.

Article.1 General rule

1. 1. Purpose,

This agreement is to comply with the law of electric communication enterprise and an Enforcement Ordinance in Korea on the utilization stipulation and procedure of all the related service provided by Macrogen Inc.

1.2. Service,

Service defines that it furnishes DNA sequencing and other additional information through to be provided by Macrogen Inc hereunder.

1.3 Effectiveness and change of the agreement,

  1. It shall come into effect on the date when Macrogen Inc. post it in public.
  2. It may be amended by any such change of important business reasons and proceeded with work as changed after all the amendments are made.

Article 2. Enrollment of a membership and Service Usage,

2.1 Eligibility and Types of accounts

  1. To be eligible for our service, you shall obtain the consent of service usage from Macrogen and make an agreement.
  2. In the event that you have the desire to create your own account and use our service, you shall provide us with your personal information in accordance with Macrogen Inc’s request
  3. In the event that Macrogen Inc. authorizes the Service Usage to you, such notice shall be considered to be received by Macrogen Inc with User ID and other related information.
  4. Macrogen Inc. does not authorize the application of our service usage in accordance with the following:
    - apply to service with name of other persons.
    - provide false, inaccurate or misleading information
    - register on purpose of a manner that is defamatory, trade libelous, unlawfully threatening or unlawfully harassing.

2.2 Service usage and limitation

  1. Macrogen Inc. can temporarily suspend the Service usage due to system inspection, change, defect, communication interruption and Force Majeure.
  2. In the event that the service usage is suspended as set forth below in clauses a), any claims of either user or third party shall be excluded.

Article 3. Liability

3.1 Macrogen Inc’s Liability

  1. Macrogen Inc. shall take a step that you can use our service immediately from the date to be registered without any failure, except for our special cases.
  2. You shall agree to receive an e-mail related to service, important notice and promotion email / letter sent by Macrogen.
  3. In the event that you escalate any claim, we will gather information from you and take a appropriate step. While it is take a few time to settle it, we will notify the reasons and schedule to you.
  4. Macrogen Inc. shall not disclose your personal information to any third party that is not directly related to the agreement and will limit to use and improve high quality service, unless the disclosure is requires by the law, regulations or orders of the governmental authorities concerned national security and safety in very exceptional case.
  5. Macrogen Inc. would not accept liability for any damage caused by natural disasters(earthquake, a war of the elements, flood, typhoon, etc).

3.2 User’s Liability

  1. You shall be liable for all the management of your own ID and Password.
  2. You shall agree to receive an e-mail related to service sent by Macrogen.
  3. You shall give the notice to Macrogen on this matters, if your ID is used on illegally purposes,
  4. You shall abide by clauses specified on the agreement and related laws.
  5. You agree to acknowledge that any data generated from Macrogen's DNA sequencing and delivered to you resulted from labour, efforts, material and assets of Macrogen and its affiliated entities, and that you shall be liable for the payments of the processed order regardless of the success or fail of the sequence data.

Article 4. Supply and usage of Service result

4.1 Supply of Service result

  1. The turnaround time define the one from the arrival of your samples to the sending of result.
  2. If the result is delayed beyond the promised date, you shall be notified in writing of the fact of delayed service.

4.2 Services Usage,

  1. Macrogen Inc. shall treat all the sample data and information provided by you confidentially and shall not disclose them to any third party without your consent, except if the disclosure is required by following purpose:
    1. provide it by anonymity for a statistical report, academic research and market investigation
    2. identity the said person to prevent use by stealth
    3. required by the laws, regulations or orders of the Governmental authorities concerned

Article 5. Effectiveness, termination and limitation

5.1 Effectiveness

  1. You shall provide your identified information as per Macrogen’s required form and make this agreement with Macrogen.
  2. Macrogen agree to register users as a member who comply with clause 5.1.a.

5.2 Termination and usage limitation

  1. You may for its convenience, terminate contract at any time. Such termination becomes effective by your e-mail notice of termination to Macrogen after identifying your personal information (Name, TEL, Institute, FAX etc)
  2. Upon receipt of the notice, you can not, except as otherwise directed by you in the notice, log in with your ID & PW and use it.
  3. Macrogen may terminate the Contract without any notice, in whole or in part, if:
    1. violate the public order and established social morals
    2. relate to criminal act
    3. intend to utilize service for damaging national interests and social public benefit.
    4. use ID and Password of other users.
    5. bring disgrace and inflict a loss on other users.
    6. register another ID in duplicate under same user.
    7. damage sound usage of service.

5.3 Cancellation procedure of usage limitation

  1. In the case of limiting service usage, Macrgoen shall notify users or representatives in writing or phone of Macrogen’s intension fixing given date and time to terminate the contract. Such termination becomes effective by KHNP's written notice of termination to Supplier.
  2. Macrogen may, for its convenience, terminate all or any part of the service at any time by urgent problem. Such termination becomes effective by Macrogen's written notice of termination to Supplier.
  3. Under the article of 4.2.1, users or representative who is notified the termination of service usage, could make a objection.
  4. In the event of resolving the suspension of service usage, Macrogen will take a appropriate action to cancel the suspension immediately.

5. 4 User’s notice management

Macrogen can delete the notice posted by users for the following without any pre-notice.

  1. injure to a persons or group’s reputation by a slander
  2. violate public order or established social morals.
  3. commit a criminal act
  4. infringe copyright against other people
  5. violate related law or Macrogen’s rule.

Article 6. Arbitration

All disputes, controversies or differences which may arise out of or in connection with the Contract or for the breach thereof shall be finally settled by arbitration in Seoul, Korea in accordance with the Commercial Arbitration Rules of the Korean Commercial Arbitration Board and the laws of Korea.

Aricle 7. Governing Law

The Contract shall be governed and interpreted by the laws of the Republic of Korea.

Article8. Liabilities and remedy

8.1 Liabilities

Unless otherwise provided herein, Supplier shall not be liable for any consequential, direct or indirect damages, related to free service , except for the damages caused by willful misconduct.
Macrogen lifetime storage service will be provided to all of our customers at free of charge up to fifteen (15) years from the date of data creation.
We are not liable for damage or losses to any of one’s standard sequencing result files which are stored in Macrogen’s server resulting from participation in or accessing or downloading file or data in connection with the Service.
We reserve the right, in our sole discretion, to cancel or suspend the Service should a virus, bugs, or other causes beyond our control corrupt the administration, security or proper operation of the Service.

8.2 Remedy

  1. Macrogen have no obligation to confirm and represent any opinion and information provided by Macrogen’ sequencing service, users and third party.
  2. Macorgen shall not be liable for any loss caused by commodity transaction or leading and borrowing money through service between users and a third party and expected profit from service.

8.3 Liquidated damage,

You shall pay liquidated damages, not as a penalty, to Macrogen in an amount of 10% of total amount of the delayed payment behind due date.

# Appendix

  1. All payment shall be made by you to the designated Macrogen’s banking account, or by the individual or corporate credit card and shall be made in the United States Dollar or Euro or Japanese Yen or British Pound.
  2. According to the general terms and conditions on above-mentioned, we would like to inform you that we will charge you the amount of the service charge as per the inserted card number unless the payment is done no later than one month after the receipt of the commercial invoice of our sequencing service.
  3. You can choose one of the payment methods among the followings.
    1. Telegraphic Transfer in advance
    2. Telegraphic Transfer at sight of the commercial invoice
    3. Payment of credit card as per your the level of the credit
    4. Banking Check
  4. Single Pass Sample Resequencing policy
    Resequencing is provided in order to verify any possibility of machine error or operator's mishandling and carried out only when
    DNA sequencing quality can be improved. Therefore, retrial request for failures owing to template preparation or composition
    will be all charged. Also, re-sending a new batch, in spite of the same sample names, will be regarded as a new order.